Alt W
Division of Theoretical Biology, Universitet Bonn, Germany.
Biofizika. 1996 Jan-Feb;41(1):169-77.
Migration of epidermal skin cells, keratinocytes, plays an important role in processes as wound healing or melanoma formation. Thus, characterization of their spontaneous motility in vitro could give indications about abnormalities of the responsible motor system, namely the cortical actomyosin layer and its interaction with the plasma membrane. Videomicroscopical observations and image data evaluations of keratinocytes spread on glass have revealed typical patterns of peripheral shape changes as rhythmical protrusions and retractions of lamellipodia, periodic waves of ruffles moving towards the cell body and, finally, pulsating or rotating waves of cell protrusions. We present two models of the behavior of actomyosin network taken as a highly viscous reactive and contractive polymer fluid. One describes the dynamics of lamellipodial protrusions transversal to the cell periphery, the other considers a simplified circular model along the whole cell circumference. Simulations of these partial differential equation models reproduce the observed spatio-temporal patterns.
表皮皮肤细胞，即角质形成细胞的迁移，在伤口愈合或黑色素瘤形成等过程中发挥着重要作用。因此，体外表征它们的自发运动性可以为相关运动系统的异常情况提供线索，即皮层肌动球蛋白层及其与质膜的相互作用。对角质形成细胞在玻璃上展开的视频显微镜观察和图像数据评估揭示了外周形状变化的典型模式，如片状伪足的有节奏的突出和缩回、向细胞体移动的周期性波状褶皱，以及最终细胞突出的脉动或旋转波。我们提出了两种将肌动球蛋白网络视为高粘性反应性和收缩性聚合物流体的行为模型。一种描述了与细胞周边横向的片状伪足突出的动力学，另一种考虑了沿整个细胞周长的简化圆形模型。这些偏微分方程模型的模拟再现了观察到的时空模式。
