大鼠松果体中表达前脑啡肽原mRNA的细胞并非产生5-羟色胺的松果体细胞：运用原位杂交结合5-羟色胺免疫细胞化学的证据。
Cells expressing preproenkephalin mRNA in the rat pineal gland are not serotonin-producing pinealocytes: evidence using in situ hybridization combined with immunocytochemistry for serotonin.
作者信息
Wang X T, Pappas G D, Sagen J, Unnerstall J R
机构信息
Department of Anatomy and Cell Biology, University of Illinois at Chicago College of Medicine 60612, USA.
出版信息
Cell Mol Neurobiol. 1996 Feb;16(1):73-84. doi: 10.1007/BF02578388.
Abstract
- Preproenkephalin (PPEnk) mRNA expressing cells have been identified in rat pineal gland using radioactive in situ hybridization histochemistry. 2. Approximately 7% of the cells in the pineal gland (7.5 +/- 0.86, mean +/- 95% CI) express PPEnk mRNA. These cells are distributed throughout the pineal as either scattered single cells or small groups of cells with large round or oval nuclei. 3. Using in situ hybridization combined with ABC immunocytochemistry for serotonin (5-HT) in the same pineal sections, the PPEnk mRNA labeling cells are found not to be serotonin-immunoreactive cells. These data indicate that the PPEnk mRNA is expressed in a certain discrete subpopulation of cells in the rat pineal gland and these cells are not serotonin-producing pinealocytes. 4. The physiologic role of PPEnk-derived peptides in the pineal remains unknown. It is possible that these peptides either are synthesized and secreted as hormones or act as pineal paracrine signals.
摘要
- 利用放射性原位杂交组织化学技术，在大鼠松果体中鉴定出了表达前脑啡肽原（PPEnk）mRNA的细胞。2. 松果体中约7%的细胞（7.5±0.86，平均值±95%可信区间）表达PPEnk mRNA。这些细胞散布于整个松果体，呈单个散在细胞或具有大的圆形或椭圆形细胞核的小细胞群。3. 在同一松果体切片中，将原位杂交与5-羟色胺（5-HT）的ABC免疫细胞化学相结合，发现PPEnk mRNA标记细胞并非5-羟色胺免疫反应性细胞。这些数据表明，PPEnk mRNA在大鼠松果体中特定的离散细胞亚群中表达，且这些细胞不是产生5-羟色胺的松果体细胞。4. PPEnk衍生肽在松果体中的生理作用尚不清楚。这些肽有可能作为激素被合成和分泌，或作为松果体旁分泌信号发挥作用。
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本文引用的文献
1
Differential extractions for the spectrophotofluorometric measurement of diverse 5-hydroxy- and 5-methoxyindoles.用于分光光度荧光法测定多种5-羟基吲哚和5-甲氧基吲哚的差异提取法。
Anal Biochem. 1963 Jan;5:51-9. doi: 10.1016/0003-2697(63)90057-5.
2
Immunocytochemical demonstration of nerve growth factor (NGF) receptor in the pineal gland: effect of NGF on pinealocyte neurite formation.
Brain Res. 1993 Apr 30;610(1):108-14. doi: 10.1016/0006-8993(93)91223-f.
3
Immunocytochemical and electron-microscopic characterization of macrophage/microglia cells and expression of class II major histocompatibility complex in the pineal gland of the rat.大鼠松果体中巨噬细胞/小胶质细胞的免疫细胞化学和电子显微镜特征以及II类主要组织相容性复合体的表达
Cell Tissue Res. 1993 May;272(2):257-65. doi: 10.1007/BF00302731.
4
The presence of nerve fibers immunoreactive for vasoactive intestinal peptide (VIP), peptide histidine isoleucine (PHI), and preproVIP(111-122) in the mouse pineal gland.
J Pineal Res. 1994 Jan;16(1):50-6. doi: 10.1111/j.1600-079x.1994.tb00082.x.
5
Immunocytochemical localization of basic fibroblast growth factor in the rat pineal gland.
J Pineal Res. 1994 Jan;16(1):44-9. doi: 10.1111/j.1600-079x.1994.tb00081.x.
6
Immunohistochemical evidence for the presence of melatonin in the pineal gland, the retina and the Harderian gland.松果体、视网膜和哈德氏腺中存在褪黑素的免疫组织化学证据。
Cell Tissue Res. 1981;217(1):105-15. doi: 10.1007/BF00233830.
7
Vasopressin- and oxytocin-containing fibres in the pineal gland and subcommissural organ of the rat.大鼠松果体和连合下器官中含加压素和催产素的纤维。
Cell Tissue Res. 1980;205(1):11-7. doi: 10.1007/BF00234438.
8
Ultrastructural characterization of glial cells in the rat pineal gland with special reference to the pineal stalk.大鼠松果体中神经胶质细胞的超微结构特征，特别提及松果体柄。
Anat Rec. 1984 Dec;210(4):663-74. doi: 10.1002/ar.1092100414.
9
Isolation and characterization of the rat proenkephalin gene.
J Biol Chem. 1984 Nov 25;259(22):14309-13.
10
Distribution of substance P neurons in the epithalamus of the rat: an immunohistochemical investigation.
J Pineal Res. 1984;1(4):355-70. doi: 10.1111/j.1600-079x.1984.tb00226.x.
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