Vivien-Roels B, Pévet P, Dubois M P, Arendt J, Brown G M
Cell Tissue Res. 1981;217(1):105-15. doi: 10.1007/BF00233830.
The presence of melatonin is demonstrated in the pineal gland, the retina and the Harderian gland in some mammalian and non-mammalian vertebrates, using a specific fluorescence labelled antibody technique. Four different potent antibodies against melatonin have been used and compared. In the pineal gland of hamsters, mice, rats and snakes, specific fluorescence, mostly restricted to the cytoplasm of the cells, is detected in pinealocytes. Fluorescence is also detected in the pineal organ of fishes, tortoises and lizards, but it has not been possible, from cryostat sections of fresh tissue, to assert which kind of cell is reacting (photoreceptor cells or interstitial ependymal cells). In the retina, fluorescence is almost exclusively restricted to the outer nuclear layer. In the Harderian gland of mammals and reptiles, fluorescence is localized in the secretory cells of the alveoli and mostly restricted to the cytoplasm surrounding the nucleus. These results are discussed in relation to the concept of melatonin synthesis at extrapineal sites independent of pineal production.
利用一种特定的荧光标记抗体技术，在一些哺乳动物和非哺乳动物脊椎动物的松果体、视网膜和哈德氏腺中证实了褪黑素的存在。已使用并比较了四种不同的抗褪黑素强效抗体。在仓鼠、小鼠、大鼠和蛇的松果体中，在松果体细胞中检测到特异性荧光，大部分局限于细胞的细胞质。在鱼类、乌龟和蜥蜴的松果体器官中也检测到荧光，但从新鲜组织的低温切片中，无法确定哪种细胞在发生反应（光感受器细胞或间质室管膜细胞）。在视网膜中，荧光几乎完全局限于外核层。在哺乳动物和爬行动物的哈德氏腺中，荧光定位于肺泡的分泌细胞，大部分局限于细胞核周围的细胞质。结合松果体以外部位独立于松果体产生的褪黑素合成概念对这些结果进行了讨论。
