Selliseth N J, Selvig K A
Department of Dental Research, Univ. of Bergen School of Dentistry, Norway.
Scanning Microsc. 1995 Jun;9(2):455-67; discussion 467-8.
The purpose of this study was to examine microvascular regeneration associated with gingival wound healing. A full-thickness piece of gingiva and oral mucosa was excised along the palatal aspect of the right maxillary first and second molars in 20 young Wistar rats. The contralateral side served as unoperated control. After 2, 4, 7, 10 or 20 days of healing, microvascular corrosion casts were produced and examined by scanning electron microscopy. At 2 days, vessels surrounding the wound were dilated and impressions representing sites of leukocyte margination were prominent in the walls of venules. Capillary buds were emerging from venules and capillaries. At 4 days, the vessel buds had lengthened and connected in pairs to produce capillary loops. At 7 days, new vessels extended deeply into the wound space, mainly from the medical side, in a palisade-like pattern. At 10 days, the denuded bone surface was still not completely revascularized and Volkman's canals opening to the wound area were empty. At 20 days, the bone surface was covered by large, irregular vessels which originated mainly from the palatal mucosa. The periodontal ligament was less important in the tissue repair process, while the bony vasculature contributed little or not at all to revascularization of the healing gingiva and palatal mucosa.
本研究的目的是检查与牙龈伤口愈合相关的微血管再生。在20只年轻的Wistar大鼠中，沿右上颌第一和第二磨牙的腭侧切除一块全层牙龈和口腔黏膜。对侧作为未手术对照。在愈合2、4、7、10或20天后，制作微血管腐蚀铸型并通过扫描电子显微镜检查。在第2天，伤口周围的血管扩张，小静脉壁上代表白细胞靠边部位的压迹明显。毛细血管芽从小静脉和毛细血管中长出。在第4天，血管芽延长并成对连接形成毛细血管环。在第7天，新血管主要从内侧以栅栏状模式深入伤口空间。在第10天，裸露的骨表面仍未完全重新血管化，通向伤口区域的Volkmann管是空的。在第20天，骨表面被主要起源于腭黏膜的大的不规则血管覆盖。牙周韧带在组织修复过程中不太重要，而骨血管系统对愈合的牙龈和腭黏膜的重新血管化贡献很小或几乎没有贡献。
