Park Y K, Yang M H, Park H R
Department of Pathology, College of Medicine, Kyung Hee University Medical Center, Seoul, Korea.
Skeletal Radiol. 1996 Jan;25(1):13-7. doi: 10.1007/s002560050025.
Thirty-three osteosarcomas at various grades of histologic differentiation, including chondroblastic, osteoblastic, and fibroblastic variants, were investigated immunohistochemically for evidence of osteonectin. Twenty-two cases of varying types of osteosarcoma were examined with in situ hybridization for mRNA expression of osteonectin. Immunohistochemically, osteonectin was present in all the osteosarcomas in this study. With in situ hybridization, 12 out of 22 osteosarcomas showed a positive signal. Two osteochondrosarcomas, seven chondrosarcomas, and one mesenchymal chondrosarcoma were also studied with regard to the localization of osteonectin, either immunohistochemically or by in situ hybridization. Immunohistochemically, osteonectin was present in all the chondroid lesions except for one osteochondroma. However, in situ hybridization of osteonectin mRNA was negative in all the chondroid lesions we studied. This study revealed that immunohistochemical localization of osteonectin is not useful in providing conclusive diagnosis of osteosarcoma. In situ hybridization of osteonectin mRNA might be useful in differentiating osteosarcoma from nonsteogenic bone tumors.
对33例不同组织学分化程度的骨肉瘤进行了免疫组织化学研究，这些骨肉瘤包括软骨母细胞型、成骨细胞型和纤维母细胞型变体，以寻找骨连接蛋白的证据。对22例不同类型的骨肉瘤进行原位杂交，检测骨连接蛋白的mRNA表达。免疫组织化学研究显示，本研究中的所有骨肉瘤均存在骨连接蛋白。原位杂交结果显示，22例骨肉瘤中有12例呈阳性信号。还对2例骨软骨肉瘤、7例软骨肉瘤和1例间叶性软骨肉瘤进行了骨连接蛋白定位研究，采用免疫组织化学或原位杂交方法。免疫组织化学研究显示，除1例骨软骨瘤外，所有软骨样病变均存在骨连接蛋白。然而，我们研究的所有软骨样病变中骨连接蛋白mRNA的原位杂交均为阴性。本研究表明，骨连接蛋白的免疫组织化学定位对骨肉瘤的确诊并无帮助。骨连接蛋白mRNA的原位杂交可能有助于骨肉瘤与非成骨性骨肿瘤的鉴别诊断。
