Detlavs I, Dombrovska L, Turauska A, Shkirmante B, Slutskii L
Latvian Medical Academy, National Hospital of Traumatology and Orthopaedics, Riga.
Sci Total Environ. 1996 Feb 2;180(1):35-42. doi: 10.1016/0048-9697(95)04917-7.
The effect of radio frequency electromagnetic fields (RF EMF) was studied on Wistar rats with excised full-thickness dermal wounds in the interscapular region. The wounded regions of experimental animals were subjected to EMF for 30 min daily during the first 5 days after wound infliction. Control animals received no treatment. We used RF EMF with (1) frequency 53.53 GHz without modulation; (2) frequency 42.19 GHz without modulation; (3) frequency 42.19 GHz, but with a frequency modulation band 200-MHz wide. On the 7th day the animals were terminated and the granulation-fibrous tissue (GFT) developed in the wounds was subjected to complex quantitative biochemical analysis. RF EMF without frequency modulation decreased the amounts of glycoprotein macromolecules, diminishing the inflammatory exudation. In striking contrast, under the influence of RF EMF with frequency modulation, hexoses and especially sialic acid concentrations were significantly elevated (P < 0.001). This indicated intensification of exudative phenomena. As a consequence of inflammation inhibition in the treatment without frequency modulation, the total collagen accumulation was lowered. However, when frequency was modulated, the inflammatory phenomena were intensified, and pronounced accumulation of collagenous proteins was noted. Thus, our experiments confirm the effects of non-thermal EMF on the reparative-proliferative processes of animals with soft tissue wounds.
研究了射频电磁场（RF EMF）对肩胛间区域全层皮肤切除伤口的Wistar大鼠的影响。在伤口造成后的前5天，每天对实验动物的伤口区域施加30分钟的电磁场。对照动物不接受治疗。我们使用了以下几种射频电磁场：（1）频率53.53GHz，无调制；（2）频率42.19GHz，无调制；（3）频率42.19GHz，但具有200MHz宽的频率调制带。在第7天处死动物，对伤口中形成的肉芽-纤维组织（GFT）进行复杂的定量生化分析。无频率调制的射频电磁场减少了糖蛋白大分子的数量，减少了炎症渗出。与之形成鲜明对比的是，在有频率调制的射频电磁场影响下，己糖尤其是唾液酸的浓度显著升高（P<0.001）。这表明渗出现象加剧。由于无频率调制治疗中炎症受到抑制，总的胶原蛋白积累量降低。然而，当进行频率调制时，炎症现象加剧，并且观察到明显的胶原蛋白积累。因此，我们的实验证实了非热电磁场对软组织伤口动物修复-增殖过程的影响。
