Maeda M
Department of Obstetrics and Gynecology, Kitasato University School of Medicine, Kanagawa, Japan.
Nihon Sanka Fujinka Gakkai Zasshi. 1996 Feb;48(2):111-7.
Effects of oxidative stress (OS) on the fetus were studied under ischemic induced by clamping the uterine vessels of pregnant rats. OS was evaluated by superoxide (O-.2) released from red blood cells. Fetal RBC-O-.2 of the ischemic rats (clamping for 10 min and 20 min) was significantly higher than that of the control group (p < 0.01). Maternal RBC-O-.2 tended to increase, but not significantly. When readings before and after clamping were compared, the activity of fetal RBC SOD had increased in the ischemic group (clamping time for 10 min), but significantly decreased in the 20 min clamping rats (p < 0.01). Maternal SOD activity tended to decrease along with clamping time. The amount of lipidperoxide was significantly greater in the fetal ischemic liver subjected to clamping for 20 minutes than in the tissue subjected to clamping for 10 minutes. The lipidperoxide level was significantly higher in the fetal ischemic brain exposed for 10 minutes than in the control. These results indicate that fetal RBC-O-.2 increased along with ischemic time, and the maternal RBC-O-.2 had been influenced by the increase in fetal RBC-O-.2. Based on these observations, it was concluded that the OS due to ischemic injury had a more adverse effect on the fetal brain than on the liver.
通过夹闭妊娠大鼠子宫血管诱导缺血，研究氧化应激（OS）对胎儿的影响。通过红细胞释放的超氧化物（O₂⁻）评估OS。缺血大鼠（夹闭10分钟和20分钟）的胎儿红细胞O₂⁻显著高于对照组（p < 0.01）。母体红细胞O₂⁻有增加趋势，但不显著。比较夹闭前后读数时，缺血组（夹闭时间10分钟）胎儿红细胞超氧化物歧化酶（SOD）活性增加，但夹闭20分钟的大鼠中显著降低（p < 0.01）。母体SOD活性随夹闭时间有降低趋势。夹闭20分钟的胎儿缺血肝脏中脂质过氧化物量显著高于夹闭10分钟的组织。暴露10分钟的胎儿缺血脑中脂质过氧化物水平显著高于对照组。这些结果表明胎儿红细胞O₂⁻随缺血时间增加，母体红细胞O₂⁻受胎儿红细胞O₂⁻增加的影响。基于这些观察结果，得出结论：缺血性损伤引起的OS对胎儿脑的不良影响大于对肝脏的影响。
