Aarts M G, Keijzer C J, Stiekema W J, Pereira A
Department of Molecular Biology, Dienst Landbouwkundig Onderzoek-Centre for Plant Breeding and Reproduction Research, Wageningen, Netherlands.
Plant Cell. 1995 Dec;7(12):2115-27. doi: 10.1105/tpc.7.12.2115.
The aerial parts of plants are coated with an epicuticular wax layer, which is important as a first line of defense against external influences. In Arabidopsis, the ECERIFERUM (CER) genes effect different steps of the wax biosynthesis pathway. In this article, we describe the isolation of the CER1 gene, which encodes a novel protein involved in the conversion of long chain aldehydes to alkanes, a key step in was biosynthesis. CER1 was cloned after gene tagging with the heterologous maize transposable element system Enhancer-Inhibitor, also known as Suppressor-mutator. cer1 mutants display glossy green stems and fruits and are conditionally male sterile. The similarity of the CER1 protein with a group of integral membrane enzymes, which process highly hydrophobic molecules, points to a function of the CER1 protein as a decarbonylase.
植物地上部分覆盖着一层角质层蜡质层，作为抵御外界影响的第一道防线，这层蜡质层很重要。在拟南芥中，蜡质合成酶（CER）基因影响蜡质生物合成途径的不同步骤。在本文中，我们描述了CER1基因的分离，该基因编码一种参与长链醛转化为烷烃的新蛋白质，这是蜡质生物合成中的关键步骤。CER1是在用异源玉米转座子系统增强子-抑制子（也称为抑制子-突变体）进行基因标签后克隆得到的。cer1突变体表现出茎和果实呈亮绿色且存在条件性雄性不育。CER1蛋白与一组处理高度疏水分子的整合膜酶的相似性表明CER1蛋白具有脱羰酶的功能。
