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[成年大鼠心肌细胞在体内培养时反应性变化的超微结构特征]

[The ultrastructural characteristics of the reactive changes in the cardiomyocytes of adult rats when cultured in vivo].

作者信息

Stadnikov A A, Dedkov E I

出版信息

Morfologiia. 1994 Apr-Jun;106(4-6):124-9.

PMID:8718644
Abstract

60 implantes of the left atrium myocardium were studied on the 1st, 3rd and 6th days of in vivo culture according to F. M. Lazarenko method (outbred laboratory male rats of 200-250g. body weight were used both as donors and recipients). Reactive changes of the cultured cardiomyocytes were assessed by methods of light and electron microscopy. Polymorphic nature of the cardiomyocyte reactive changes with the preservation of its tissue pecific determination was established. Myocardium implant did not form myosymplastic elements, cellular characteristics of organization remained in its implanted pieces. Data concerning degenerating and surviving cardiomyocytes are presented as well as those on cellular forms, identification of which is difficult on the ultrastructural level.

摘要

根据F.M.拉扎连科方法,在体内培养的第1天、第3天和第6天对60个左心房心肌植入物进行了研究(使用体重200 - 250克的远交系实验室雄性大鼠作为供体和受体)。通过光学显微镜和电子显微镜方法评估培养的心肌细胞的反应性变化。确定了心肌细胞反应性变化的多形性本质及其组织特异性特征的保留。心肌植入物未形成肌联合元件,其植入块中仍保留细胞组织特征。还呈现了关于退化和存活心肌细胞的数据以及细胞形态的数据,这些细胞形态在超微结构水平上难以识别。

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