Gretzer C, Eriksson A S, Alldén B, Ericson L E, Thomsen P
Institute of Anatomy and Cell Biology, Gothenburg University, Sweden.
Biomaterials. 1996 May;17(9):851-8. doi: 10.1016/0142-9612(96)83279-0.
The release of interleukin-1 alpha (IL-1 alpha) by human peripheral blood monocytes cultured for 24 and 48 h on polystyrene (PS) and titanium-sputtered polystyrene (Ti) was evaluated. Magnetron sputtering of the PS surfaces resulted in a formation of a 50-nm-thick coat, consisting of an outer layer of TiO2. Monocytes released IL-1 alpha without the addition of exogenous stimuli. A doubling of the culture time from 24 to 48 h did not have a major effect on the amount of IL-1 alpha released. The IL-1 alpha levels were increased by addition of lipopolysaccharide (LPS). High concentrations of PS particles (1 and 3 microns diameter) were equally effective stimuli for IL-1 alpha release as LPS. Preadsorption of fibronectin to culture plates augmented LPS-stimulated IL-1 alpha secretion, whereas preadsorbed fibrinogen had an inhibitory effect. Our observation indicate a direct activation of monocytes by PS and Ti, resulting in IL-1 alpha secretion, which is modified by protein adsorption and exogenous stimuli.
评估了人外周血单核细胞在聚苯乙烯（PS）和钛溅射聚苯乙烯（Ti）上培养24小时和48小时后白细胞介素-1α（IL-1α）的释放情况。对PS表面进行磁控溅射形成了一层50纳米厚的涂层，其外层为TiO2。单核细胞在不添加外源性刺激的情况下释放IL-1α。培养时间从24小时加倍至48小时对IL-1α的释放量没有重大影响。添加脂多糖（LPS）可提高IL-1α水平。高浓度的PS颗粒（直径1和3微米）作为IL-1α释放的刺激物与LPS同样有效。纤连蛋白预吸附到培养板上可增强LPS刺激的IL-1α分泌，而预吸附的纤维蛋白原则具有抑制作用。我们的观察结果表明PS和Ti可直接激活单核细胞，导致IL-1α分泌，这种分泌会因蛋白质吸附和外源性刺激而发生改变。
