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炎性细胞对超薄片层无定形及结晶羟基磷灰石表面的反应。

Inflammatory cell response to ultra-thin amorphous and crystalline hydroxyapatite surfaces.

机构信息

Department of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Box 412, Gothenburg, SE-405 30, Sweden.

BIOMATCELL, VINN Excellence Center of Biomaterials and Cell Therapy, Gothenburg, Sweden.

出版信息

J Mater Sci Mater Med. 2017 Jan;28(1):9. doi: 10.1007/s10856-016-5814-2. Epub 2016 Nov 28.

DOI:10.1007/s10856-016-5814-2
PMID:27896599
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5126180/
Abstract

It has been suggested that surface modification with a thin hydroxyapatite (HA) coating enhances the osseointegration of titanium implants. However, there is insufficient information about the biological processes involved in the HA-induced response. This study aimed to investigate the inflammatory cell response to titanium implants with either amorphous or crystalline thin HA. Human mononuclear cells were cultured on titanium discs with a machined surface or with a thin, 0.1 μm, amorphous or crystalline HA coating. Cells were cultured for 24 and 96 h, with and without lipopolysaccharide (LPS) stimulation. The surfaces were characterized with respect to chemistry, phase composition, wettability and topography. Biological analyses included the percentage of implant-adherent cells and the secretion of pro-inflammatory cytokine (TNF-α) and growth factors (BMP-2 and TGF-β1). Crystalline HA revealed a smooth surface, whereas the amorphous HA displayed a porous structure, at nano-scale, and a hydrophobic surface. Higher TNF-α secretion and a higher ratio of adherent cells were demonstrated for the amorphous HA compared with the crystalline HA. TGF-β1 secretion was detected in all groups, but without any difference. No BMP-2 secretion was detected in any of the groups. The addition of LPS resulted in a significant increase in TNF-α in all groups, whereas TGF-β1 was not affected. Taken together, the results show that thin HA coatings with similar micro-roughness but a different phase composition, nano-scale roughness and wettability are associated with different monocyte responses. In the absence of strong inflammatory stimuli, crystalline hydroxyapatite elicits a lower inflammatory response compared with amorphous hydroxyapatite.

摘要

有人提出,通过在钛植入物表面进行羟基磷灰石(HA)涂层处理,可以增强其与骨的整合。然而,目前对于 HA 诱导反应所涉及的生物学过程的信息还不够充分。本研究旨在探究具有非晶态或结晶态的 HA 薄涂层的钛植入物的炎症细胞反应。将人类单核细胞培养在经过机械加工的钛片表面或具有 0.1μm 厚的非晶态或结晶态 HA 涂层的钛片上。细胞分别在 24 小时和 96 小时时进行培养,同时有或没有脂多糖(LPS)刺激。通过化学、相组成、润湿性和形貌对表面进行了特征描述。生物学分析包括附着在植入物上的细胞百分比和促炎细胞因子(TNF-α)和生长因子(BMP-2 和 TGF-β1)的分泌。结晶态 HA 呈现出光滑的表面,而非晶态 HA 则呈现出纳米级多孔结构和疏水性表面。与结晶态 HA 相比,非晶态 HA 显示出更高的 TNF-α分泌和更高的附着细胞比例。所有组均检测到 TGF-β1 的分泌,但没有差异。在任何一组中都未检测到 BMP-2 的分泌。加入 LPS 后,所有组的 TNF-α 均显著增加,而 TGF-β1 不受影响。综上所述,结果表明,具有相似微观粗糙度但不同相组成、纳米级粗糙度和润湿性的 HA 薄涂层与不同的单核细胞反应相关。在没有强烈炎症刺激的情况下,与非晶态羟基磷灰石相比,结晶态羟基磷灰石会引起较低的炎症反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fcc/5126180/c2ed481bba67/10856_2016_5814_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fcc/5126180/d8b2d5f5f8b4/10856_2016_5814_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fcc/5126180/7dca60836085/10856_2016_5814_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fcc/5126180/e7d62163a8e0/10856_2016_5814_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fcc/5126180/c2ed481bba67/10856_2016_5814_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fcc/5126180/d8b2d5f5f8b4/10856_2016_5814_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fcc/5126180/7dca60836085/10856_2016_5814_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fcc/5126180/e7d62163a8e0/10856_2016_5814_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fcc/5126180/c2ed481bba67/10856_2016_5814_Fig4_HTML.jpg

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