den Braber E T, de Ruijter J E, Smits H T, Ginsel L A, von Recum A F, Jansen J A
University of Nijmegen, Dental School, Department of Oral Function, The Netherlands.
Biomaterials. 1996 Jun;17(11):1093-9. doi: 10.1016/0142-9612(96)85910-2.
In order to quantify the effect of the substrata surface topography on cellular behaviour, planar and micro-textured silicon substrata were produced and made suitable for cell culture by radio frequency glow discharge treatment. These substrata possessed parallel surface grooves with a groove and ridge width of 2.0 (SilD02), 5.0 (SilD05) and 10 microns (SilD10). Groove depth was approximately 0.5 micron. Rat dermal fibroblasts (RDFs) were cultured on these substrata and a tissue culture polystyrene control surface for 1, 2, 3, 5 and 7 days. After incubation the cell proliferation was quantified with a Coulter Counter, and RDF size, shape and orientation with digital image analysis. Cell counts proved that neither the presence of the surface grooves nor the dimension of these grooves had an effect on the cell proliferation. However, RDFs on SilD02, and to a lesser extent on SilD05 substrata, were elongated and aligned parallel to the surface grooves. Orientation of the RDFs on SilD10 substrata proved to be almost comparable to the SilD00 substrata. Finally, it was observed that the cells on the micro-textured substrata were capable of spanning the surface grooves.
为了量化基质表面形貌对细胞行为的影响，制备了平面和微纹理化的硅基质，并通过射频辉光放电处理使其适合细胞培养。这些基质具有平行的表面凹槽，凹槽和脊的宽度分别为2.0微米（SilD02）、5.0微米（SilD05）和10微米（SilD10）。凹槽深度约为0.5微米。将大鼠真皮成纤维细胞（RDFs）在这些基质以及组织培养聚苯乙烯对照表面上培养1、2、3、5和7天。孵育后，用库尔特计数器对细胞增殖进行量化，并用数字图像分析RDF的大小、形状和取向。细胞计数证明，表面凹槽的存在及其尺寸对细胞增殖均无影响。然而，SilD02上的RDFs，以及在较小程度上SilD05基质上的RDFs，被拉长并与表面凹槽平行排列。事实证明，SilD10基质上RDFs的取向与SilD00基质几乎相当。最后，观察到微纹理化基质上的细胞能够跨越表面凹槽。
