den Braber E T, de Ruijter J E, Smits H T, Ginsel L A, von Recum A F, Jansen J A
Department of Oral Function, Dental School, University of Nijmegen, The Netherlands.
J Biomed Mater Res. 1995 Apr;29(4):511-8. doi: 10.1002/jbm.820290411.
To evaluate the effect of surface treatment and surface microtexture on cellular behavior, smooth and microtextured silicone substrata were produced. The microtextured substrata possessed parallel surface grooves with a width and spacing of 2.0 (SilD02), 5.0 (SilD05), and 10 microns (SilD10). The groove depth was approximately 0.5 microns. Subsequently, these substrata were either left untreated (NT) or treated by ultraviolet irradiation (UV), radiofrequency glow discharge treatment (RFGD), or both (UVRFGD). After characterization of the substrata, rat dermal fibroblasts (RDF) were cultured on the UV, RFGD, and UVRFGD treated surfaces for 1, 3, 5, and 7 days. Comparison between the NT and UV substrata revealed that UV treatment did not influence the contact angles and surface energies of surfaces with a similar surface topography. However, the contact angles of the RFGD and UVRFGD substrata were significantly smaller than those of the UV and NT substrata. The dimension of the surface microevents did not influence the wettability characteristics. Cell culture experiments revealed that RDF cell growth on UV-treated surfaces was lower than on the RFGD and UVRFGD substrata. SEM examination demonstrated that the parallel surface grooves on the SilD02 and SilD05 substrata were able to induce stronger cell orientation and alignment than the events on SilD10 surfaces. By combining all of our findings, the most important conclusion was that physicochemical parameters such as wettability and surface free energy influence cell growth but play no measurable role in the shape and orientation of cells on microtextured surfaces.
为评估表面处理和表面微观纹理对细胞行为的影响，制备了光滑和具有微观纹理的硅酮基质。具有微观纹理的基质具有宽度和间距为2.0微米（SilD02）、5.0微米（SilD05）和10微米（SilD10）的平行表面凹槽。凹槽深度约为0.5微米。随后，这些基质要么不进行处理（NT），要么通过紫外线照射（UV）、射频辉光放电处理（RFGD）或两者结合（UVRFGD）进行处理。在对基质进行表征后，将大鼠真皮成纤维细胞（RDF）在经UV、RFGD和UVRFGD处理的表面上培养1、3、5和7天。NT和UV基质之间的比较表明，UV处理不会影响具有相似表面形貌的表面的接触角和表面能。然而，RFGD和UVRFGD基质的接触角明显小于UV和NT基质的接触角。表面微观事件的尺寸不影响润湿性特征。细胞培养实验表明，RDF细胞在UV处理表面上的生长低于在RFGD和UVRFGD基质上的生长。扫描电子显微镜检查表明，SilD02和SilD05基质上的平行表面凹槽比SilD10表面上的事件能够诱导更强的细胞定向和排列。综合我们所有的研究结果，最重要的结论是，诸如润湿性和表面自由能等物理化学参数会影响细胞生长，但在微观纹理表面上对细胞的形状和定向没有可测量的作用。
