Engelhardt J K, Ishikawa K, Mori J, Shimabukuro Y
Brain Res. 1977 Jun 10;128(2):243-8. doi: 10.1016/0006-8993(77)90991-x.
The passive electrical properties of cultured chick skeletal muscle are significantly altered when the muscle is co-cultured with spinal cord explants. A reduced transverse membrane resistance appears to be responsible for the smaller values of input resistance, space constant, and time constant observed in co-cultures relative to those observed in pure muscle cultures. In this report, we show that neuromuscular junctions are not required in order to observe this neurotrophic effect because medium from spinal cord explant cultures is capable of producing the same reduction in transverse membrane resistance as the co-culturing of spinal cord explants with muscle. Control medium from liver explant cultures has no effect on muscle passive electrical properties. These results indicate that a trophic substance which is capable of regulating the electrical properties of excitable cells is released into the culture medium by spinal cord explants.
当培养的鸡骨骼肌与脊髓外植体共培养时，其被动电特性会发生显著改变。横向膜电阻降低似乎是导致共培养物中观察到的输入电阻、空间常数和时间常数相对于纯肌肉培养物中观察到的值更小的原因。在本报告中，我们表明观察到这种神经营养作用并不需要神经肌肉接头，因为脊髓外植体培养物的培养基能够产生与脊髓外植体与肌肉共培养相同程度的横向膜电阻降低。来自肝脏外植体培养物的对照培养基对肌肉的被动电特性没有影响。这些结果表明，脊髓外植体将一种能够调节可兴奋细胞电特性的营养物质释放到培养基中。
