Jackson G F, Villablanca J R, Loopuijt L D
Department of Psychiatry, UCLA School of Medicine, USA.
Brain Res Dev Brain Res. 1995 Dec 21;90(1-2):62-72. doi: 10.1016/0165-3806(96)83487-0.
To further understand the neuroanatomical consequences following perinatal brain injury, quantitative morphometric analysis was performed on the brain of cats receiving a unilateral frontal cortical ablation between postnatal days (P) 9 and P 14 and intact control cats. In all cats, the volume of the neocortex and thalamus was measured bilaterally and that of the thalamic ventrobasal complex (VBc) was measured ipsilaterally. In addition, using stereology, the neuronal and glial (presumably) cell packing densities (CPD) and the total number of neurons and glial cells (TCN) were measured in the ipsilateral VBc. The neuronal and glial cell cross-sectional areas (CSA) were also measured in the ipsilateral VBc. The mean ipsilateral and contralateral neocortex volumes were similar between the two animal groups. There was a statistically significant 14% and 13% reduction in mean ipsilateral and contralateral thalamic volumes, respectively for the lesioned animals, while the VBc shrank by 16% relative to intact controls. The mean neuronal and glial CPD were similar between the two groups. The mean neuronal TCN was reduced by 10% in the neonatal-lesioned cats, while the mean glial TCN was reduced by 31% in the same animals, however neither value reached significance. Lastly, the mean CSA of neurons and glial cells showed a tendency to be smaller in the lesioned cats by 8% and 9%, respectively. These results: (a) indicate that the neonatal lesion caused only minor morphological brain alterations and this sharply contrast with the marked changes previously reported in cats with a similar lesion sustained prenatally; (b) suggest that the enhanced behavioral recovery and/or sparing reported for the present cats compared to fetal-lesioned animals is at least partially due to the morphological sparing reported here; (c) together with previous findings in fetal cats, support the hypothesis that the morphological changes after a neonatal neocortical lesion are qualitatively different and may depend on different mechanisms as compared to those occurring after similar damage sustained prenatally.
为了进一步了解围产期脑损伤后的神经解剖学后果，对出生后第9天至第14天接受单侧额叶皮质切除的猫以及完整对照猫的大脑进行了定量形态计量分析。在所有猫中，双侧测量新皮质和丘脑的体积，同侧测量丘脑腹侧基底复合体（VBc）的体积。此外，使用体视学方法，测量同侧VBc中神经元和神经胶质（可能）细胞填充密度（CPD）以及神经元和神经胶质细胞总数（TCN）。还测量了同侧VBc中神经元和神经胶质细胞的横截面积（CSA）。两组动物的同侧和对侧新皮质平均体积相似。损伤动物的同侧和对侧丘脑平均体积分别有统计学意义地减少了14%和13%，而VBc相对于完整对照缩小了16%。两组之间的平均神经元和神经胶质CPD相似。新生期损伤的猫中，平均神经元TCN减少了10%，而同一动物中平均神经胶质TCN减少了31%，但两者均未达到统计学意义。最后，损伤猫中神经元和神经胶质细胞的平均CSA分别有缩小8%和9%的趋势。这些结果：（a）表明新生期损伤仅引起轻微的脑形态学改变，这与先前报道的产前遭受类似损伤的猫的明显变化形成鲜明对比；（b）表明与胎儿期损伤动物相比，本研究中猫所报道的行为恢复增强和/或保留至少部分归因于此处所报道的形态学保留；（c）与先前对胎儿猫的研究结果一起，支持这样的假设，即新生期新皮质损伤后的形态学变化在性质上是不同的，并且与产前遭受类似损伤后发生的变化可能依赖于不同的机制。
