与成年猫大脑半球切除术后相比，新生猫大脑半球切除术后丘脑变性较少。

There is less thalamic degeneration in neonatal-lesioned than in adult-lesioned cats after cerebral hemispherectomy.

作者信息

Villablanca J R, Burgess J W, Benedetti F

出版信息

Brain Res. 1986 Mar 19;368(2):211-25. doi: 10.1016/0006-8993(86)90564-0.

Abstract

In order to study age-related processes of degeneration and recovery, the left hemitelencephalon was surgically removed in 5 adult cats and 5 neonatal kittens which were compared to 5 intact controls. After long survival, brains were sectioned in the coronal plane and thionine-stained. Drawings of gross and microscopic thalamic structures were made at 4 planes and computer-digitized to provide 7 measures: total thalamic area at planes A 7.5 and A 8.5; counts of neuroglia, small neurons (31-100 microns2) and large neurons (101-1000 microns2); area of neuroglia, small neurons and large neurons. All cellular measurements were in the ventrobasal complex at planes A 8.0 and A 10.0. Morphological changes were found bilaterally in all lesioned cats. Ipsilateral to the ablation in adult-lesioned cats, thalamic area, large neuron count and glia cells size were markedly and significantly decreased (P less than 0.01), while in neonatal-lesioned cats these changes were present but significantly reduced in magnitude compared to adult-lesioned animals. In addition, adult-lesioned cats showed a marked increase in glial cell numbers (P less than 0.01) and a decrease in small neuron size (P less than 0.01), while kitten-lesioned animals did not show changes in these measures. In the intact side of the brain, the thalamus of adult-lesioned cats was decreased in size (P less than 0.01), and glial cells were decreased in number and size (P less than 0.05), while in kitten-lesioned brains there were few changes. In both lesioned groups large neurons showed a significant increase in size (P less than 0.01). We conclude that neonatal hemispherectomy results in markedly less thalamic atrophy, retrograde neuronal degeneration and gliosis than the equivalent lesion in adults. The changes are discussed in the context of the increased neuroanatomical reorganization and functional recovery which were reported in neonatal- vs adult-lesioned animals.

摘要

为了研究与年龄相关的退化和恢复过程，对5只成年猫和5只新生小猫进行手术切除左侧半端脑，并与5只完整对照进行比较。经过长时间存活后，将大脑在冠状平面切片并用硫堇染色。在4个平面绘制丘脑大体和微观结构的图，并进行计算机数字化处理以提供7项测量指标：A 7.5和A 8.5平面的丘脑总面积；神经胶质细胞、小神经元（31 - 100平方微米）和大神经元（101 - 1000平方微米）的计数；神经胶质细胞、小神经元和大神经元的面积。所有细胞测量均在A 8.0和A 10.0平面的腹侧基底复合体中进行。在所有受损猫中均发现双侧形态学变化。在成年受损猫中，与切除同侧相比，丘脑面积、大神经元计数和神经胶质细胞大小显著减少（P < 0.01），而在新生受损猫中这些变化存在，但与成年受损动物相比程度明显减轻。此外，成年受损猫的神经胶质细胞数量显著增加（P < 0.01），小神经元大小减小（P < 0.01），而新生受损动物在这些指标上没有变化。在成年受损猫大脑的完整侧，丘脑大小减小（P < 0.01），神经胶质细胞数量和大小减少（P < 0.05），而在新生受损大脑中变化很少。在两个受损组中，大神经元大小均显著增加（P < 0.01）。我们得出结论，与成年动物的同等损伤相比，新生半球切除术导致的丘脑萎缩、逆行性神经元变性和神经胶质增生明显更少。这些变化在新生与成年受损动物中报道的神经解剖学重组增加和功能恢复的背景下进行了讨论。