Haehling E, Niederkorn J Y, Stewart G L
Center for Parasitology, University of Texas at Arlington 76019, USA.
Int J Parasitol. 1995 Dec;25(12):1393-400. doi: 10.1016/0020-7519(95)00080-1.
Immunoperoxidase staining of muscle infected with Trichinella spiralis for murine collagen types I and IV provided both qualitative and quantitative evidence of extensive synthesis of both types of collagen by fibroblasts in infected muscle compared to that seen in uninfected muscle. Moreover, fibroblasts in muscle infected with T. pseudospiralis, a nonencapsulating species, showed significantly less staining for both types of collagen compared to muscle from mice infected with T. spiralis. Analysis of collagen composition of isolated nurse cells using an ELISA specific for either type I or type IV murine collagen suggested that of these 2 types of collagen, only type IV basement membrane collagen is found in Trichinella capsular collagen. Excretory/secretory products of T. spiralis and T. pseudospiralis induced extensive synthesis of exclusively type IV collagen by 3T3 murine fibroblasts in vitro.
用免疫过氧化物酶法对感染旋毛虫的小鼠肌肉进行Ⅰ型和Ⅳ型胶原蛋白染色，结果表明，与未感染肌肉相比，感染肌肉中的成纤维细胞大量合成这两种胶原蛋白，在定性和定量方面都有证据支持。此外，感染伪旋毛虫（一种无包囊的旋毛虫）的小鼠肌肉中的成纤维细胞，与感染旋毛虫的小鼠肌肉相比，这两种胶原蛋白的染色明显较少。使用针对Ⅰ型或Ⅳ型小鼠胶原蛋白的ELISA对分离出的保姆细胞的胶原蛋白组成进行分析，结果表明，在旋毛虫包囊胶原蛋白中，这两种胶原蛋白中只有Ⅳ型基底膜胶原蛋白。旋毛虫和伪旋毛虫的排泄/分泌产物在体外诱导3T3小鼠成纤维细胞大量合成仅Ⅳ型胶原蛋白。
