MacDiarmid J A, Clarke R, McClure S J, Bowen F R, Burrell D H
CSIRO Division of Animal Health, McMaster Laboratory, NSW, Australia.
Int J Parasitol. 1995 Dec;25(12):1505-7. doi: 10.1016/0020-7519(95)00027-5.
A new monoclonal raised against sheep IgE was used to examine sera and wound exudates from sheep which had been struck by Lucilia cuprina in the field. The antibody was also used to detect the presence of IgE in sera and skin sections from sheep which had been artificially infected with fly larvae 3 times. Neither total, nor L. cuprina specific circulating IgE could be detected in serum or wound exudates from struck sheep. Cell bound IgE was, however, identified by the monoclonal in skin sections from struck sheep and from a control sheep which had not been struck. No difference in the number of IgE positive cells was observed between the control and 2 of the 3 artificially infected sheep, and none of the latter showed an increase in IgE positive cells even after 3 infections. One sheep showed twice as many IgE positive cells as the other treated sheep and the third larval infection was difficult to establish and limited in size and severity. This suggests a relationship between innate resistance to strike and the number of IgE positive cells present in skin.
一种新制备的抗绵羊IgE单克隆抗体被用于检测在野外被铜绿蝇侵袭的绵羊的血清和伤口渗出液。该抗体还被用于检测人工感染蝇幼虫3次的绵羊的血清和皮肤切片中IgE的存在情况。在被侵袭绵羊的血清或伤口渗出液中，既未检测到总的循环IgE，也未检测到铜绿蝇特异性循环IgE。然而，通过该单克隆抗体在被侵袭绵羊和未被侵袭的对照绵羊的皮肤切片中鉴定出了细胞结合型IgE。在对照绵羊和3只人工感染绵羊中的2只之间，未观察到IgE阳性细胞数量的差异，并且即使在3次感染后，后者中也没有一只显示出IgE阳性细胞增加。一只绵羊的IgE阳性细胞数量是其他处理过的绵羊的两倍，并且第三次幼虫感染难以建立，规模和严重程度有限。这表明对侵袭的先天抵抗力与皮肤中存在的IgE阳性细胞数量之间存在关联。
