Colditz I G, Eisemann C H, Tellam R L, McClure S J, Mortimer S I, Husband A J
CSIRO Division of Animal Production, Pastoral Research Laboratory, Armidale, NSW 2350, Australia.
Int J Parasitol. 1996 Jul;26(7):775-82. doi: 10.1016/0020-7519(96)00048-3.
Intensive lymphocytic infiltration of the underlying dermis occurs during cutaneous myiasis caused by larvae of the blow fly, Lucilia cuprina. To determine the effect of this infiltrate on larval growth, monoclonal antibodies (mAb) to CD4, CD8 or WC1 lymphocyte subset determinants were injected intravenously before and during experimental infection of sheep with larvae. The effect of intravenous injection of mAb to ovine interferon (IFN) gamma was also examined. The experiments were performed in 18-month-old maiden ewes with genetic resistance or susceptibility to the disease complex, bacterial dermatitis/cutaneous myiasis. mAbs induced profound depletion of CD8+ and WC1+ subpopulations from blood and skin at sites of larval growth. mAb to CD4+ gave only a moderate reduction in lymphocytes from blood or skin. mAb treatments did not modify larval growth or survival at 20 or 50 h after infection. Larval growth rates did not differ between resistant and susceptible genotypes. No evidence was found for a role of T lymphocyte subpopulations or the cytokine IFN, in modifying larval growth during the first 50 h of infection. It seems unlikely that T lymphocyte-dependent immunological effector mechanisms contribute to the lower prevalence of fly strike seen in the resistant genotype in the field.
由绿蝇 Lucilia cuprina 的幼虫引起的皮肤蝇蛆病期间，真皮深层会出现密集的淋巴细胞浸润。为了确定这种浸润对幼虫生长的影响，在绵羊被幼虫实验性感染之前和期间，静脉注射针对 CD4、CD8 或 WC1 淋巴细胞亚群决定簇的单克隆抗体（mAb）。还检测了静脉注射抗绵羊干扰素（IFN）γ 单克隆抗体的效果。实验在 18 月龄、对疾病复合体（细菌性皮炎/皮肤蝇蛆病）具有遗传抗性或易感性的未孕母羊中进行。单克隆抗体导致幼虫生长部位血液和皮肤中 CD8 + 和 WC1 + 亚群显著减少。针对 CD4 + 的单克隆抗体仅使血液或皮肤中的淋巴细胞适度减少。单克隆抗体处理并未改变感染后 20 小时或 50 小时时幼虫的生长或存活情况。抗性和易感基因型之间的幼虫生长速率没有差异。未发现 T 淋巴细胞亚群或细胞因子 IFN 在感染后最初 50 小时内改变幼虫生长方面发挥作用。在野外抗性基因型中看到的较低的蝇蛆侵袭发生率，似乎不太可能是由 T 淋巴细胞依赖性免疫效应机制导致的。
