Baek N I, Kim D S, Lee Y H, Park J D, Lee C B, Kim S I
Korea Ginseng & Tobacco Research Institute, Taejeon, Korea.
Planta Med. 1996 Feb;62(1):86-7. doi: 10.1055/s-2006-957816.
A genuine glycoside, named ginsenoside Rh4, was isolated from Korean red ginseng (Panax ginseng C. A. Meyer) through repeated column chromatography, and its chemical structure was established to be 6-O-beta-D-glucopyranosyldammar-20(22),24-diene-3 beta,6 alpha,12 beta-triol by spectral and chemical methods. The stereochemistry of a double bond at C-20(22) of ginsenoside Rh4 was characterized as (E) from a NOESY experiment in the 1H-NMR of the aglycone. Cytotoxic activities of ginsenoside Rh4 and its aglycone against cancer cell lines were evaluated by use of the SRB method.
从高丽红参（人参C. A. 迈耶）中通过反复柱色谱法分离得到一种真正的糖苷，命名为人参皂苷Rh4，并通过光谱和化学方法确定其化学结构为6-O-β-D-吡喃葡萄糖基达玛烷-20(22),24-二烯-3β,6α,12β-三醇。通过苷元的1H-NMR中的NOESY实验，人参皂苷Rh4在C-20(22)处双键的立体化学特征为(E)型。采用SRB法评估了人参皂苷Rh4及其苷元对癌细胞系的细胞毒性活性。
