Healy D J, Meador-Woodruff J H
Mental Health Research Institute, University of Michigan, Ann Arbor 48109-0720, USA.
Brain Res. 1996 Feb 5;708(1-2):38-44. doi: 10.1016/0006-8993(95)01241-9.
Glutamate agonists have been shown to stimulate striatal dopamine release, but less is known about dopamine-glutamate interactions at the receptor level. We treated rats with 0.3, 1.0, or 3.0 mg/kg of MK-801, an NMDA antagonist, daily for 1 week and, using in situ hybridization, measured dopamine receptor mRNA levels in cortical and subcortical structures. MK-801 caused a significant increase of D1 and D2 mRNA in the dorsal and ventral striatum, a significant decrease of D3 mRNA in the nucleus accumbens, and a significant decrease of D1 mRNA in the limbic cortex. Dopamine autoreceptor expression, reflected by D2 mRNA in the midbrain, was increased in the ventral tegmental area, but not in the substantia nigra. Thus, MK-801 appears to differentially regulate the mesocorticolimbic and nigrostriatal dopamine systems.
谷氨酸激动剂已被证明能刺激纹状体多巴胺释放，但在受体水平上，多巴胺与谷氨酸之间的相互作用却鲜为人知。我们每天给大鼠注射0.3、1.0或3.0毫克/千克的NMDA拮抗剂MK-801，持续1周，然后利用原位杂交技术测量皮质和皮质下结构中多巴胺受体mRNA水平。MK-801导致背侧和腹侧纹状体中D1和D2 mRNA显著增加，伏隔核中D3 mRNA显著减少，边缘皮质中D1 mRNA显著减少。中脑D2 mRNA所反映的多巴胺自身受体表达在腹侧被盖区增加，但在黑质中未增加。因此，MK-801似乎对中脑边缘多巴胺系统和黑质纹状体多巴胺系统有不同的调节作用。
