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在耐青霉素肺炎链球菌的青霉素结合蛋白2B基因中检测到9核苷酸序列的直接重复插入。

Directly repeated insertion of 9-nucleotide sequence detected in penicillin-binding protein 2B gene of penicillin-resistant Streptococcus pneumoniae.

作者信息

Yamane A, Nakano H, Asahi Y, Ubukata K, Konno M

机构信息

Institute for Biotechnology Research, Wakunaga Pharmaceutical Co., Ltd., Hiroshima, Japan.

出版信息

Antimicrob Agents Chemother. 1996 May;40(5):1257-9. doi: 10.1128/AAC.40.5.1257.

DOI:10.1128/AAC.40.5.1257
PMID:8723477
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC163302/
Abstract

We investigated the molecular mechanism of 50 penicillin-resistant Streptococcus pneumoniae strains (penicillin: MIC, > or = 0.125 microgram/ml) having neither class A nor class B mutations in the penicillin-binding protein 2B gene (pbp2b). An analysis of the nucleotide sequences of the pbp2b genes from seven strains revealed an unique direct repeat of 9 nucleotides (TGGTATACT) between active-site serine (residue 385) and Ser-X-Asn (residues 442 to 444) motifs. The same insertion was detected in 13 strains.

摘要

我们研究了50株对青霉素耐药的肺炎链球菌菌株（青霉素：最低抑菌浓度，≥0.125微克/毫升）的分子机制，这些菌株在青霉素结合蛋白2B基因（pbp2b）中既没有A类突变也没有B类突变。对7株菌株的pbp2b基因核苷酸序列分析显示，在活性位点丝氨酸（第385位残基）和Ser-X-Asn（第442至444位残基）基序之间存在一个独特的9个核苷酸的直接重复序列（TGGTATACT）。在13株菌株中检测到相同的插入序列。

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本文引用的文献

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Evolution of penicillin resistance in Streptococcus pneumoniae; the role of Streptococcus mitis in the formation of a low affinity PBP2B in S. pneumoniae.肺炎链球菌中青霉素耐药性的演变；缓症链球菌在肺炎链球菌低亲和力青霉素结合蛋白2B形成中的作用。
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Alterations in penicillin-binding protein 2B from penicillin-resistant wild-type strains of Streptococcus pneumoniae.肺炎链球菌青霉素耐药野生型菌株中青霉素结合蛋白2B的改变。
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Genetics of high level penicillin resistance in clinical isolates of Streptococcus pneumoniae.肺炎链球菌临床分离株中高水平青霉素耐药性的遗传学研究
FEMS Microbiol Lett. 1995 Mar 1;126(3):299-303. doi: 10.1111/j.1574-6968.1995.tb07433.x.
4
Occurrence of a beta-lactam-inducible penicillin-binding protein in methicillin-resistant staphylococci.耐甲氧西林葡萄球菌中β-内酰胺诱导型青霉素结合蛋白的出现。
Antimicrob Agents Chemother. 1985 May;27(5):851-7. doi: 10.1128/AAC.27.5.851.
5
Diagnosis of sickle cell anemia and beta-thalassemia with enzymatically amplified DNA and nonradioactive allele-specific oligonucleotide probes.利用酶促扩增DNA和非放射性等位基因特异性寡核苷酸探针诊断镰状细胞贫血和β地中海贫血。
N Engl J Med. 1988 Sep 1;319(9):537-41. doi: 10.1056/NEJM198809013190903.
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