在耐青霉素肺炎链球菌的青霉素结合蛋白2B基因中检测到9核苷酸序列的直接重复插入。
Directly repeated insertion of 9-nucleotide sequence detected in penicillin-binding protein 2B gene of penicillin-resistant Streptococcus pneumoniae.
作者信息
Yamane A, Nakano H, Asahi Y, Ubukata K, Konno M
机构信息
Institute for Biotechnology Research, Wakunaga Pharmaceutical Co., Ltd., Hiroshima, Japan.
出版信息
Antimicrob Agents Chemother. 1996 May;40(5):1257-9. doi: 10.1128/AAC.40.5.1257.
Abstract
We investigated the molecular mechanism of 50 penicillin-resistant Streptococcus pneumoniae strains (penicillin: MIC, > or = 0.125 microgram/ml) having neither class A nor class B mutations in the penicillin-binding protein 2B gene (pbp2b). An analysis of the nucleotide sequences of the pbp2b genes from seven strains revealed an unique direct repeat of 9 nucleotides (TGGTATACT) between active-site serine (residue 385) and Ser-X-Asn (residues 442 to 444) motifs. The same insertion was detected in 13 strains.
摘要
我们研究了50株对青霉素耐药的肺炎链球菌菌株（青霉素：最低抑菌浓度，≥0.125微克/毫升）的分子机制，这些菌株在青霉素结合蛋白2B基因（pbp2b）中既没有A类突变也没有B类突变。对7株菌株的pbp2b基因核苷酸序列分析显示，在活性位点丝氨酸（第385位残基）和Ser-X-Asn（第442至444位残基）基序之间存在一个独特的9个核苷酸的直接重复序列（TGGTATACT）。在13株菌株中检测到相同的插入序列。
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