Deuschl H, Johansson S G
Clin Allergy. 1977 Mar;7(2):195-202. doi: 10.1111/j.1365-2222.1977.tb01442.x.
Nasal secretions from eighteen patients with allergic rhinitis with a positive case history, intradermal test and nasal provocation test, but with negative or only weakly positive RAST (radioallergosorbent test) on the serum against a total of thirty-five allergens, were studied. In the RAST an immunosorbent-purified anti-IgE with DE2 specificity was used, which raised the detection limit. Nasal secretion was collected by washing the nasal mucosa with 0-9% and 18% NaCl solution respectively, and the latter secretion was also luophilized and concentrated. In ten cases RAST was slightly positive on the nasal secretion, and in three of the concentrated secretions the RAST value was higher than on the serum. In none of the serum or nasal secretion samples was RAST positive according to the cut off value for a positive result defined by the reference system used in Phadebas Rast. From these results it is concluded that RAST analyses of nasal secretion from patients with allergic rhinitis is of no appreciable value in routine clinical allergological diagnosis. However, the increased sensitivity of RAST obtained with isotope-labelled anti-DE2 may be useful in the serological diagnosis of patients with low grade allergy having low levels of IgE antibodies in serum.
对18例过敏性鼻炎患者的鼻分泌物进行了研究。这些患者有阳性病史、皮内试验和鼻激发试验阳性，但血清中针对总共35种变应原的放射性变应原吸附试验（RAST）呈阴性或仅弱阳性。在RAST中，使用了具有DE2特异性的免疫吸附纯化抗IgE，提高了检测限。分别用0.9%和1.8%的氯化钠溶液冲洗鼻粘膜收集鼻分泌物，后一种分泌物也进行了冻干和浓缩。10例鼻分泌物的RAST呈弱阳性，3例浓缩分泌物的RAST值高于血清。根据Phadebas Rast所用参考系统定义的阳性结果临界值，血清或鼻分泌物样本均无RAST阳性。从这些结果可以得出结论，过敏性鼻炎患者鼻分泌物的RAST分析在常规临床变态反应诊断中没有明显价值。然而，用同位素标记的抗DE2获得的RAST敏感性增加，可能有助于血清学诊断血清中IgE抗体水平低的轻度过敏患者。
