用于同时测量血红蛋白色素的二极管阵列分光光度法。
Diode-array spectrophotometry for simultaneous measurement of hemoglobin pigments.
作者信息
Fogh-Andersen N, Siggaard-Andersen O, Lundsgaard F C, Wimberley P D
出版信息
Clin Chim Acta. 1987 Jul 15;166(2-3):283-9. doi: 10.1016/0009-8981(87)90431-1.
A prototype oxygen saturation meter was used to measure the concentrations of deoxygenated hemoglobin (rHb), oxyhemoglobin (HbO2), carboxyhemoglobin (HbCO), methemoglobin (MetHb), and sulfhemoglobin (SHb) in 35 microliter blood. Simultaneous absorbance measurements at 535, 560, 577, 622, 636, and 670 nm permitted the composition of any hemoglobin pigment mixture to be determined more accurately, precisely and easily than before. The inclusion of 670 nm, where the hemoglobin pigments have low absorption coefficients, allowed correction for turbidity.
使用一台原型血氧饱和度计测量35微升血液中脱氧血红蛋白（rHb）、氧合血红蛋白（HbO2）、碳氧血红蛋白（HbCO）、高铁血红蛋白（MetHb）和硫化血红蛋白（SHb）的浓度。在535、560、577、622、636和670纳米处同时进行吸光度测量，使得能够比以往更准确、精确且轻松地确定任何血红蛋白色素混合物的组成。纳入血红蛋白色素吸收系数较低的670纳米波长，可对浊度进行校正。
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