Lam R W, Artal R, Fisher D A
Clin Chem. 1977 Jul;23(7):1264-7.
We describe a convenient and specific radioimmunoassay for urinary metanephrine, in which we used an antiserum generated in rabbits by injecting with synephrine conjugated to bovine serum albumin as described by Grota and Brown [Endocrinology 98, 615 (1976)]. The antiserum is specific for metanephrine and epinephrine. Epinephrine cross reaction is minimized to 1% by adding 2 ng of epinephrine to each assay tube. Incubation time is 12 h. The sensitivity of the assay is 40 pg. Within-and between-assay coefficients of variation are 7.0 and 9.7%, respectively. We assayed first-voided morning urine and 24-h urine specimens from 15 normal subjects before and after acid hydrolysis, to assess total and free metanephrine concentrations. Mean (+/-SD) total and free urinary metanephrine excretion were 17 +/- 11 and 3.5 +/- 1.0 micrograms/24 h in these subjects; corresponding values for the morning specimens were 13.4 +/- 8.3 and 3.5 +/- 1.5 micrograms/liter, respectively.
我们描述了一种便捷且特异的尿间甲肾上腺素放射免疫测定法，其中我们使用了如Grota和Brown [《内分泌学》98, 615 (1976)]所述的，通过用与牛血清白蛋白偶联的辛弗林注射兔而产生的抗血清。该抗血清对间甲肾上腺素和肾上腺素具有特异性。通过向每个测定管中加入2 ng肾上腺素，可将肾上腺素交叉反应最小化至1%。孵育时间为12小时。该测定法的灵敏度为40 pg。批内和批间变异系数分别为7.0%和9.7%。我们对15名正常受试者酸水解前后的晨尿和24小时尿液标本进行了检测，以评估总间甲肾上腺素和游离间甲肾上腺素浓度。这些受试者尿总间甲肾上腺素和游离间甲肾上腺素的平均（±标准差）排泄量分别为17±11和3.5±1.0微克/24小时；晨尿标本的相应值分别为13.4±8.3和3.5±1.5微克/升。
