一种使用高效液相色谱-荧光检测法测定尿中变肾上腺素的简单方法。
A simple method for the assay of urinary metanephrines using high performance liquid chromatography with fluorescence detection.
作者信息
Jackman G P
出版信息
Clin Chim Acta. 1982 Mar 26;120(1):137-42. doi: 10.1016/0009-8981(82)90084-5.
A method is described which extends a high performance liquid chromatographic assay for urinary catecholamines to the assay of urinary metanephrines. The amines are separated from urine after acid hydrolysis of conjugates by ion-exchange chromatography, and then further purified by solvent extraction. The final extracts are suitable for direct HPLC assay using the endogenous fluorescence of the amines for detection. Twenty-four hour excretion of the amines in 35 hospital in-patients was found to be (mean and range: 169 micrograms/24 h normetanephrine (39-423), 102 micrograms/24 h metanephrine (19-290) and 138 micrograms/24 h 3-methoxytyramine (32-234)).
本文描述了一种方法，该方法将用于尿儿茶酚胺的高效液相色谱测定法扩展至尿甲氧基肾上腺素的测定。通过离子交换色谱法在对结合物进行酸水解后将胺类从尿液中分离出来，然后通过溶剂萃取进一步纯化。最终提取物适用于直接高效液相色谱测定，利用胺类的内源性荧光进行检测。在35名住院患者中发现，这些胺类的24小时排泄量分别为（平均值及范围：去甲变肾上腺素169微克/24小时（39 - 423），变肾上腺素102微克/24小时（19 - 290），3 - 甲氧基酪胺138微克/24小时（32 - 234））。
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