Ahern T E, Bird R C, Bird A E, Wolfe L G
Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849, USA.
Am J Vet Res. 1996 May;57(5):693-6.
To determine, for canine mammary tumors, whether malignancy, with or without local invasion or regional metastasis, was associated with overexpression of the oncogene c-erbB-2.
c-erbB-2 expression was measured in canine mammary tumor-derived cell lines and in mammary tumor tissues from clinical cases. Clinical samples were examined histologically to determine whether they were benign or malignant and, if malignant, whether they had evidence of local invasion or regional metastasis. Canine fibroblast cultures and normal canine mammary epithelial tissues were used as reference standards for cell lines and mammary tumors, respectively. SAMPLE POPULATIONS: 28 canine mammary tumor tissue samples obtained surgically from clinical cases and samples from 7 canine mammary tumor cell lines derived from primary canine mammary tumors.
c-erbB-2 mRNA levels were determined by means of hybridization of total polysomal RNA with a 32P-labeled human c-erbB-2 probe on dot blots, and results were quantified by means of scanning densitometry. Overexpression of c-erbB-2 was defined as an autoradiographic density > or = 2 times the density of reference samples on the same blot.
Overexpression of c-erbB-2 was detected in 17 of 23 malignant tumors, 0 of 5 benign tumors, and 2 of 7 mammary tumor cell lines. c-erbB-2 overexpression was correlated with a histopathologic diagnosis of malignancy (P = 0.005) but not with the presence of local invasion or regional metastatic disease (P = 0.621).
Results suggest that overexpression of c-erbB-2 occurs prior to the development of metastatic disease in canine mammary tumors and plays a role in the development of malignancy.
确定犬乳腺肿瘤的恶性程度（无论有无局部浸润或区域转移）是否与癌基因c-erbB-2的过表达有关。
检测犬乳腺肿瘤来源的细胞系以及临床病例的乳腺肿瘤组织中的c-erbB-2表达。对临床样本进行组织学检查，以确定它们是良性还是恶性，以及如果是恶性的，是否有局部浸润或区域转移的证据。犬成纤维细胞培养物和正常犬乳腺上皮组织分别用作细胞系和乳腺肿瘤的参考标准。
从临床病例手术获取的28个犬乳腺肿瘤组织样本以及来自原发性犬乳腺肿瘤的7个犬乳腺肿瘤细胞系的样本。
通过在斑点印迹上用32P标记的人c-erbB-2探针与总多核糖体RNA杂交来测定c-erbB-2 mRNA水平，并通过扫描光密度法对结果进行定量。c-erbB-2的过表达定义为放射自显影片密度≥同一印迹上参考样本密度的2倍。
在23个恶性肿瘤中的17个、5个良性肿瘤中的0个以及7个乳腺肿瘤细胞系中的2个中检测到c-erbB-2过表达。c-erbB-2过表达与恶性的组织病理学诊断相关（P = 0.005），但与局部浸润或区域转移性疾病的存在无关（P = 0.621）。
结果表明，c-erbB-2的过表达发生在犬乳腺肿瘤转移疾病发展之前，并在恶性肿瘤的发展中起作用。
