Banda P W, Sherry A E, Blois M S
Clin Chem. 1977 Aug;23(8):1397-401.
We describe recent developments in the use of a stable free radical, diphenylpicrylhydrazyl, as a colorimetric detector of reducing metabolites in melanoma urines. A procedure for buffering aqueous/solvent reagent solutions is presented, and examples of the baseline stability achieved for reference chromatograms and urine samples are provided. Chromatograms of phaeomelanin precursors and of an extract of a highly pigmented hamster melanoma are also presented. Identities are tentatively assigned for some of the chromatographic peaks that have previously been correlated with disease, including isomers of cysteinyldopa, and observations of new pigment- and tumor-related metabolites in the chromatograms are noted.
我们描述了使用稳定自由基二苯基苦味酰基肼作为黑色素瘤尿液中还原代谢物比色检测器的最新进展。介绍了缓冲水相/溶剂试剂溶液的方法，并提供了参考色谱图和尿液样品所达到的基线稳定性示例。还展示了褐黑素前体和高色素仓鼠黑色素瘤提取物的色谱图。对一些先前与疾病相关的色谱峰进行了初步鉴定，包括半胱氨酰多巴的异构体，并记录了色谱图中与色素和肿瘤相关的新代谢物。
