Yamada K, Walsh N, Hara H, Jimbow K, Chen H, Ito S
Division of Dermatology and Cutaneous Sciences, Faculty of Medicine, University of Alberta, Edmonton, Canada.
Arch Dermatol. 1992 Apr;128(4):491-4.
This article introduces a rapid high-performance liquid chromatographic assay to measure urinary pheomelanin and eumelanin metabolites, 5-S-cysteinyldopa and indoles, 5(6)-hydroxy-6(5)-methoxyindole-2-carboxylic acid.
Our high-performance liquid chromatographic study clearly showed (1) urine of melanoma patients with positive metastasis revealed significant amounts of 5-S-cysteinyldopa and indoles (5,6-dihydroxyindole-2-carboxylic acid plus 6-hydroxy-5-methoxyindole-2-carboxylic acid) above 1 mumol/d and 2 mumol/d, respectively; and (2) in patients with metastasis-free melanoma these melanin metabolites might be excreted into the urine but always less than the two values cited above.
As there is a discrepancy regarding the specificity of 5-S-cysteinyldopa as a marker for estimation of melanoma metastasis, high-performance liquid chromatographic measurement of urinary indoles will provide an additional assay in the detection of melanoma metastasis from an early stage. Both melanoma markers were increased in the urine of patients with metastatic melanoma.
本文介绍了一种快速高效液相色谱分析法，用于测定尿中褐黑素和真黑素代谢产物、5-S-半胱氨酰多巴和吲哚、5(6)-羟基-6(5)-甲氧基吲哚-2-羧酸。
我们的高效液相色谱研究清楚地表明：(1) 发生转移的黑色素瘤患者尿液中，5-S-半胱氨酰多巴和吲哚（5,6-二羟基吲哚-2-羧酸加6-羟基-5-甲氧基吲哚-2-羧酸）的含量分别显著高于1 μmol/d和2 μmol/d；(2) 在无转移的黑色素瘤患者中，这些黑色素代谢产物可能会排泄到尿液中，但始终低于上述两个值。
由于5-S-半胱氨酰多巴作为评估黑色素瘤转移标志物的特异性存在差异，高效液相色谱法测定尿中吲哚将为早期检测黑色素瘤转移提供一种额外的检测方法。两种黑色素瘤标志物在转移性黑色素瘤患者的尿液中均升高。
