Tascini C, Baldelli F, Monari C, Retini C, Pietrella D, Francisci D, Bistoni F, Vecchiarelli A
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
AIDS. 1996 May;10(5):477-83. doi: 10.1097/00002030-199605000-00005.
To investigate the effect of human recombinant interleukin (hrIL)-4 or hrIL-10 on the functional status of polymorphonuclear leukocytes (PMNL) from normal subjects and HIV-infected patients.
In an in vitro system we studied the effect of hrIL-4 or hrIL-10 on phagocytosis, fungicidal activity and superoxide anion production by PMNL.
PMNL were treated in vitro with hrIL-4 or hrIL-10 or their combination for 6 h and then candidacidal activity was evaluated in a colony-forming unit inhibition assay. Superoxide anion generation by PMNL was measured in the presence or absence of preopsonized zymosan or Candida albicans.
Treatment in vitro with hrIL-4 or hrIL-10 of PMNL for 6 h was able to impair candidacidal activity of neutrophils in both normal or HIV-infected patients. The inhibitory effect was time- and dose-dependent and was more evident in PMNL from HIV-infected subjects, and reflected in these latter cells a decrease of superoxide anion generation. The impairment of candidacidal activity in PMNL from HIV-infected patients was accompanied by survival of the yeasts shown by budding formation into phagosomic organelles of cytokine-treated PMNL.
Our data highlight new biological effects of IL-4 and IL-10 evidenced by suppressed effector function of neutrophils; this phenomenon is emphasized in HIV-infected patients suggesting a role for these cytokines in mediating increased susceptibility to microbial infection during AIDS progression.
研究人重组白细胞介素（hrIL）-4 或 hrIL-10 对正常受试者及 HIV 感染患者多形核白细胞（PMNL）功能状态的影响。
在体外系统中，我们研究了 hrIL-4 或 hrIL-10 对 PMNL 的吞噬作用、杀真菌活性及超氧阴离子产生的影响。
将 PMNL 在体外分别用 hrIL-4 或 hrIL-10 或二者联合处理 6 小时，然后在集落形成单位抑制试验中评估其杀念珠菌活性。在存在或不存在预调理酵母聚糖或白色念珠菌的情况下，测定 PMNL 产生超氧阴离子的情况。
体外将 PMNL 用 hrIL-4 或 hrIL-10 处理 6 小时，能够损害正常或 HIV 感染患者中性粒细胞的杀念珠菌活性。这种抑制作用具有时间和剂量依赖性，在 HIV 感染受试者的 PMNL 中更为明显，并且在这些细胞中表现为超氧阴离子产生减少。HIV 感染患者 PMNL 杀念珠菌活性的损害伴随着酵母的存活，表现为细胞因子处理的 PMNL 的吞噬细胞器中出现出芽形成。
我们的数据突出了 IL-4 和 IL-10 的新生物学效应，表现为中性粒细胞效应功能受到抑制；在 HIV 感染患者中这种现象更为突出，提示这些细胞因子在艾滋病进展过程中介导对微生物感染易感性增加方面发挥作用。
