Myers M J, Farrell D E, Evock-Clover C M, Cope C V, Henderson M, Steele N C
Animal Biology Branch, US FDA, Beltsville, MD 20705, USA.
Pathobiology. 1995;63(5):283-7. doi: 10.1159/000163962.
The effect of dietary chromium picolinate (CrP) and recombinant porcine growth hormone, somatotropin (rPST) administration on growth performance and cytokine production in Landrace-Poland China gilts was determined using a 2 by 2 treatment array. Treatments were: (1) control (basal diet), (2) CrP-supplemented diet (basal diet + 300 micrograms Cr3+/kg diet as CrP), (3) rPST (100 pg/kg body weight/day), and (4) rPST+CrP. CrP-supplemented diets were fed beginning at 20 kg body weight through 90 kg. Administration of rPST was begun at 60 kg weight and continued through 90 kg. All rPST treated pigs demonstrated improvements in growth performance versus controls. Pigs given CrP-supplemented diets showed no differences in growth performance. At 90 kg, pigs were challenged with endotoxin (lipopolysaccharide, 0.2 microgram/kg i.v.). Blood samples were collected at 0, 1, and 3 h postchallenge. Plasma IL-6 levels increased from 23 U/ml at time 0 to 1,927 U/ml at 3 h for control swine. Swine from the CrP treatment group had IL-6 levels of 8,130 U/ml at 3 h post-LPS. There were no differences in plasma IL-6 from pigs in the rPST and rPST+CrP treatment groups compared to the controls. Endotoxin challenge had no effect on either blood glucose levels or induction of TNF-alpha in any treatment group. PBMC from CrP-treated animals produced more IL-2 than peripheral blood mononuclear cells from all other groups.
采用2×2处理组合，测定了日粮添加吡啶甲酸铬（CrP）和注射重组猪生长激素（rPST）对长白-大约克夏后备母猪生长性能和细胞因子产生的影响。处理方式如下：（1）对照组（基础日粮）；（2）添加CrP的日粮（基础日粮+300微克Cr³⁺/千克日粮，以CrP形式添加）；（3）rPST（100皮克/千克体重/天）；（4）rPST+CrP。从体重20千克开始至90千克，饲喂添加CrP的日粮。rPST从体重达到60千克开始注射，持续至90千克。与对照组相比，所有接受rPST处理的猪生长性能均有所改善。日粮添加CrP的猪生长性能无差异。90千克时，给猪静脉注射内毒素（脂多糖，0.2微克/千克）。在注射后0、1和3小时采集血样。对照组猪血浆IL-6水平从0小时的23 U/ml升高至3小时的1927 U/ml。CrP处理组猪在注射LPS后3小时IL-6水平为8130 U/ml。与对照组相比，rPST和rPST+CrP处理组猪的血浆IL-6无差异。内毒素刺激对任何处理组的血糖水平或TNF-α诱导均无影响。CrP处理组动物的外周血单核细胞（PBMC）产生的IL-2比所有其他组的外周血单核细胞更多。
