Cancela L M, Volosin M, Molina V A
Departamento de Farmacología, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
Brain Res Bull. 1996;40(2):105-10. doi: 10.1016/0361-9230(96)00040-8.
The major goal of this study was to evaluate the influence of gangliosides (GANG) treatment on the onset of adaptive changes and the sequelae induced by stress exposure. With this purpose, the behavioral response to 5-methoxy-N,N-dimethyltryptamine (5-MeODMT, 5 mg/kg, IP) and motor activity were evaluated in rats previously submitted either to a single restraint session (2 h) or to a daily restraint event for 3 consecutive days, combined or not to GANG administration (30 mg/kg IP). GANG was always injected 2 h before stress exposure. In addition, differences in body weights were recorded throughout the experiments. A similar behavioral response after 5-MeODMT was observed between saline (SAL) and GANG unstressed rats. Exposure to one or three restraint sessions did not modify the behavioral response to 5-MeODMT, whereas the association of GANG and stress during 3 consecutive days enhanced forepaw treading and hindlimb abduction. SAL-treated animals submitted to a single or to three stressful stimuli showed reduced locomotion and rearing. The combination of GANG and stress for 3 days, but not after a unique association, reversed the decrease on motor activity induced by the aversive experience. The decrease of body weights produced by one or three stress sessions was recovered only in animals treated with GANG and stress for 3 days. These findings suggest that GANG may accelerate the onset of adaptive changes on 5-HT1 sites and attenuate certain sequelae induced by previous stress experience.
本研究的主要目的是评估神经节苷脂（GANG）治疗对应激暴露诱导的适应性变化的起始以及后遗症的影响。为此，在预先接受单次束缚2小时或连续3天每日束缚的大鼠中，评估对5-甲氧基-N，N-二甲基色胺（5-MeODMT，5mg/kg，腹腔注射）的行为反应和运动活动，同时联合或不联合给予GANG（30mg/kg腹腔注射）。GANG总是在应激暴露前2小时注射。此外，在整个实验过程中记录体重差异。生理盐水（SAL）处理的未应激大鼠和GANG处理的未应激大鼠在给予5-MeODMT后观察到相似的行为反应。暴露于一次或三次束缚不会改变对5-MeODMT的行为反应，而连续3天GANG与应激联合则增强了前爪踩踏和后肢外展。接受单次或三次应激刺激的SAL处理动物的运动和竖毛行为减少。GANG与应激联合3天（而非单次联合后）可逆转厌恶经历诱导的运动活动减少。仅在接受GANG与应激联合3天处理的动物中，一次或三次应激导致的体重下降得以恢复。这些发现表明，GANG可能加速5-HT1位点适应性变化的起始，并减轻先前应激经历诱导的某些后遗症。
