Gizatullina Z Z, Sukocheva O A, Gagel'gans A I
Biokhimiia. 1996 Mar;61(3):445-50.
The influence of the hydrocortisone (GC) different doses on rat's thymocytes energetic metabolism was investigated. It has been demonstrated that as the prolonged action of GC in vivo (20 micrograms/kg of mass during 6 d. daily) as the preincubation of thymocytes with 10 microM GC in vitro are cytotoxic, leading to absolute uncoupling of oxidative phosphorylation (OP) and decreasing the rate of DNP-stimulated respiration speed. The injection of GC (20 micrograms/kg mass) at once before 72 hours to killing resulted the OP uncoupling almost on 2,5 time. Short-time action of GC at the same dose before 3 hours to drilling and so the thymocytes preincubation with 1 microM GC stimulated the transport processes on the level of substrate oxidation without the OP uncoupling. Short-time action of GC in vivo caused the inhibition of respiration, if we used glucose as the oxidative substrate, and stimulated the respiration in a presence of pyruvate Na in incubation media.
研究了氢化可的松（GC）不同剂量对大鼠胸腺细胞能量代谢的影响。结果表明，GC在体内的长期作用（每天20微克/千克体重，持续6天）以及胸腺细胞在体外与10微摩尔/升GC预孵育均具有细胞毒性，导致氧化磷酸化（OP）完全解偶联，并降低DNP刺激的呼吸速度。在处死前72小时立即注射GC（20微克/千克体重），使OP解偶联几乎增加了2.5倍。在处死前3小时以相同剂量进行GC的短期作用，以及胸腺细胞与1微摩尔/升GC预孵育，刺激了底物氧化水平上的转运过程，而没有OP解偶联。如果我们使用葡萄糖作为氧化底物，GC在体内的短期作用会导致呼吸抑制，而在孵育培养基中存在丙酮酸钠的情况下会刺激呼吸。
