Iudina T G, Salamakha O V, Olekhnovich E V, Rogatykh N P, Egorov N S
Mikrobiologiia. 1992 Jul-Aug;61(4):577-84.
The influence of the carbon source on biological activity and morphological properties of the parasporal crystals of Bacillus thuringiensis subsp. kurstaki cultivated on three different media has been studied. Biological activity of delta-endotoxins contained in crystals was tested according to their impact on insects and microorganisms. Variations in carbon sources composition resulted in changes of biological activity and morphology of crystals, caused by alterations in synthesis rate of various delta-endotoxins contained in the crystals. Variations in the content of a certain carbon source (e.g. starch) in the medium produced no effect on the crystals properties, and the content variations of the delta-endotoxins content was proportional to the producer biomass. Control of the antibacterial effect of delta-endotoxins allows a valid evaluation of their biological activity under various growth conditions as well as comparison of the biological potential of entomopathogenic chemicals for plant protection containing delta-endotoxins with different specificity of insecticidal activity.
研究了碳源对苏云金芽孢杆菌库尔斯塔克亚种在三种不同培养基上培养时伴孢晶体的生物活性和形态特性的影响。根据晶体中所含δ-内毒素对昆虫和微生物的影响来测试其生物活性。碳源组成的变化导致晶体生物活性和形态的改变，这是由晶体中所含各种δ-内毒素合成速率的变化引起的。培养基中某一碳源（如淀粉）含量的变化对晶体特性没有影响，且δ-内毒素含量的变化与产生菌生物量成正比。控制δ-内毒素的抗菌作用有助于有效评估其在各种生长条件下的生物活性，以及比较含有具有不同杀虫活性特异性的δ-内毒素的植物保护昆虫病原化学物质的生物潜力。
