Tadjore M, Trabelsi F, Hélie R, Bergeron R, Lavoie J M
Département d'Education physique, Université de Montréal. Québec.
Arch Physiol Biochem. 1996;104(1):2-7. doi: 10.1076/apab.104.1.2.12868.
The purpose of the present study was to determine the effects of an acute hepatic vagotomy on plasma insulin and catecholamines levels in fasted and fed rats with and without adrenodemedullation. Rats were first divided into two groups: adrenodemedullated (ADM) and non-adrenodemedullated rats, each group being subdivided into a normally fed and a fasted (14 hrs) group. Anesthetized rats were first ADM or sham operated, and a jugular catheter was inserted. The first blood sample was taken 30 min later (time 0). Rats were then hepatic vagotomized (HV) or sham operated, and the second blood sample was taken 30 min after the HV. Results (0 vs 30 min) indicate an increase in plasma glucose, epinephrine, and norepinephrine concentrations in rats non-adrenodemedullated. This effect was independent of the nutritional state and the integrity of the vagus nerve. A weakly significant (P < 0.07) effect of the HV on insulin response was found in ADM rats, independently of the fed or fast state (different levels of hepatic glycogen content). These results indicate the necessity of using ADM animals to study the effects of an acute HV. They also suggest that the hepato-pancreatic axis is not influenced by the level of hepatic glycogen.
本研究的目的是确定急性肝迷走神经切断术对禁食和进食的去肾上腺髓质及未去肾上腺髓质大鼠血浆胰岛素和儿茶酚胺水平的影响。大鼠首先被分为两组：去肾上腺髓质（ADM）组和未去肾上腺髓质组，每组再细分为正常进食组和禁食（14小时）组。对麻醉的大鼠首先进行去肾上腺髓质或假手术，并插入颈静脉导管。30分钟后采集第一份血样（时间0）。然后对大鼠进行肝迷走神经切断术（HV）或假手术，并在HV术后30分钟采集第二份血样。结果（0分钟与30分钟对比）表明，未去肾上腺髓质的大鼠血浆葡萄糖、肾上腺素和去甲肾上腺素浓度升高。这种效应与营养状态和迷走神经的完整性无关。在ADM大鼠中发现，无论处于进食或禁食状态（肝糖原含量水平不同），HV对胰岛素反应均有微弱显著影响（P < 0.07）。这些结果表明，有必要使用去肾上腺髓质的动物来研究急性肝迷走神经切断术的影响。它们还表明，肝-胰轴不受肝糖原水平的影响。
