Trabelsi F, Hélie R, Lavoie J M
Département d'éducation physique, Université de Montréal, Québec, Canada.
Physiol Behav. 1995 Dec;58(6):1111-5. doi: 10.1016/0031-9384(95)02051-9.
The present investigation was designed to evaluate the effect of a selective hepatic vagotomy (HV) on the insulin response in rats fasted for 24 h when blood glucose levels were or were not maintained by a constant glucose infusion. Rats were divided into three dietary groups: one group of normally fed rats, one group of 24-h fasted rats, and one group of 24-h fasted rats infused with glucose throughout the fasting period. Each of these groups was subdivided into HV and sham-operated (SHM) rats. Fasting without glucose infusion resulted in a significant (p < 0.05) decrease in plasma glucose, liver glycogen, and insulin concentrations and in a significant (p < 0.01) increase in beta-hydroxybutyrate and FFA concentration. Despite the maintenance of plasma glucose concentrations in the glucose-infused groups, the concentrations of liver glycogen and insulin were still decreased (p < 0.01) and the concentrations of beta-hydroxybutyrate were still increased (p<0.05) at the end of the fasting period. However, no significant differences in insulin or in beta-hydroxybutyrate concentration were found between HV and SHM rats. It is concluded that the decline in plasma glucose concentration during fasting does not totally explain the insulinopenic response to fasting, and that the liver, through the mediation of the hepatic vagus nerve, does not seem to contribute to insulinopenia in 24-h fasted rats.
本研究旨在评估选择性肝迷走神经切断术（HV）对禁食24小时的大鼠胰岛素反应的影响，这些大鼠在血糖水平通过持续输注葡萄糖维持或未维持的情况下进行实验。大鼠被分为三个饮食组：一组正常喂养的大鼠，一组禁食24小时的大鼠，以及一组在禁食期间持续输注葡萄糖的禁食24小时的大鼠。每组又分为HV组和假手术（SHM）组大鼠。未输注葡萄糖的禁食导致血浆葡萄糖、肝糖原和胰岛素浓度显著降低（p < 0.05），β-羟基丁酸酯和游离脂肪酸（FFA）浓度显著升高（p < 0.01）。尽管在输注葡萄糖的组中维持了血浆葡萄糖浓度，但在禁食期结束时，肝糖原和胰岛素浓度仍降低（p < 0.01），β-羟基丁酸酯浓度仍升高（p < 0.05）。然而，HV组和SHM组大鼠之间在胰岛素或β-羟基丁酸酯浓度方面未发现显著差异。研究得出结论，禁食期间血浆葡萄糖浓度的下降并不能完全解释对禁食的胰岛素分泌减少反应，并且肝脏通过肝迷走神经的介导，似乎对禁食24小时的大鼠胰岛素减少没有作用。
