Chon C H, Yao X Y, Dalal R, Takeuchi A, Kim R Y, Marmor M F
Department of Ophthalmology, Stanford University School of Medicine, California, USA.
Retina. 1996;16(2):139-44. doi: 10.1097/00006982-199616020-00010.
The authors document an animal model of retinal pigment epithelial (RPE) detachments.
N-ethylmaleimide (NEM) and sodium iodate (sulfhydryl-alkylating agents) were injected into the vitreous of Dutch pigmented rabbits. Subretinal fluid samples were withdrawn 0.5 and 3 hours after NEM injections and analyzed for albumin content by gel electrophoresis. Selected eyes were examined histologically.
Injections of 9.2 micrograms NEM caused wrinkling of the retina within 15 minutes, at which time fluorescein angiography revealed multifocal RPE detachments. At this stage, histologic sections showed moderate choroidal edema and RPE detachment. Elevated retinal detachments developed over the next 15 to 25 minutes. Histologic sections showed massive choroidal edema and RPE fragmentation. The albumin concentration of subretinal fluid rose from 60% of serum level at 30 minutes after NEM to 80% after 3 hours. Sodium iodate did not cause choroidal edema or RPE detachment.
In rabbits, intravitreal NEM causes the rapid appearance of choroidal edema and RPE detachment, followed by serous retinal detachment. The initial effect probably is caused by an alteration of choroidal vascular permeability. The relationship of these effects to sulfhydryl alkylation is unclear because sodium iodate failed to produce RPE detachments. N-ethylmaleimide effects may model aspects of clinical RPE and serous retinal detachments.
作者记录一种视网膜色素上皮（RPE）脱离的动物模型。
将N - 乙基马来酰亚胺（NEM）和碘酸钠（巯基烷基化剂）注入荷兰有色兔的玻璃体。在注射NEM后0.5小时和3小时抽取视网膜下液样本，通过凝胶电泳分析白蛋白含量。对选定的眼睛进行组织学检查。
注射9.2微克NEM在15分钟内导致视网膜起皱，此时荧光素血管造影显示多灶性RPE脱离。在此阶段，组织学切片显示中度脉络膜水肿和RPE脱离。在接下来的15至25分钟内出现视网膜脱离加重。组织学切片显示大量脉络膜水肿和RPE破碎。视网膜下液的白蛋白浓度从NEM注射后30分钟时血清水平的60%上升至3小时后的80%。碘酸钠未引起脉络膜水肿或RPE脱离。
在兔中，玻璃体内注射NEM会导致脉络膜水肿和RPE脱离迅速出现，随后出现浆液性视网膜脱离。最初的效应可能是由脉络膜血管通透性改变引起的。由于碘酸钠未能产生RPE脱离，这些效应与巯基烷基化的关系尚不清楚。N - 乙基马来酰亚胺效应可能模拟临床RPE和浆液性视网膜脱离的某些方面。
