Mark H F, Naram R, Singer J, Rice R, LaMarche P H
Department of Pathology, Rhode Island Hospital, Providence 02903, USA.
Ann Clin Lab Sci. 1996 May-Jun;26(3):269-74.
The present study applies the approach described in Mark et al. for the testing of toxic chemicals produced during the drying of Douglas-fir. The genotoxic potential of Douglas-fir condensate has been previously unexplored and is thus an area of appropriate concern to the forest products industry, regulatory, agencies, and the general public. Previous research conducted in this laboratory has identified two wood-drying condensates that yield positive cytotoxic and genotoxic effects. The results of testing Southern yellow pine and Eastern white pine condensates have been reported elsewhere. Douglas-fir condensate, a third wood-drying condensate, was added in vitro in concentrations ranging from 0.1 to 100 microliters/ml to cultures of Chinese Hamster Ovary (CHO-WBL) cells. A dose response curve was observed with this condensate for both cytotoxicity and genotoxicity. The number of viable cells as well as the mitotic index (MI) and proliferative rate index (PRI) varied inversely with dosage. The result of chromosome aberration (Abs) analysis and sister chromatid exchange (SCE) analysis, both cytogenetic measures of genotoxicity, also gave statistically significant results.
本研究采用了马克等人所描述的方法，用于测试花旗松干燥过程中产生的有毒化学物质。花旗松冷凝物的遗传毒性潜力此前尚未得到探索，因此是林产品行业、监管机构和公众适当关注的领域。本实验室此前进行的研究已经确定了两种木材干燥冷凝物，它们会产生阳性细胞毒性和遗传毒性作用。南方黄松和东部白松冷凝物的测试结果已在其他地方报道。第三种木材干燥冷凝物——花旗松冷凝物，以0.1至100微升/毫升的浓度体外添加到中国仓鼠卵巢（CHO-WBL）细胞培养物中。观察到该冷凝物对细胞毒性和遗传毒性均呈现剂量反应曲线。活细胞数量以及有丝分裂指数（MI）和增殖率指数（PRI）与剂量呈反比。染色体畸变（Abs）分析和姐妹染色单体交换（SCE）分析的结果，这两种遗传毒性的细胞遗传学测量方法，也给出了具有统计学意义的结果。
