Mark H F, Naram R, Bastan W C, Cherkes J K, LaMarche P H
Laboratory of Cytogenetics, FISH and Genotoxicology, Rhode Island Hospital, Providence, USA.
Cytobios. 1995;84(338-339):133-40.
A major activity of the lumber industry is the kiln-drying of wood. In order to ascertain whether wood-drying condensates pose a possible environmental hazard, the cytotoxicity and genotoxicity of these condensates in vitro, were tested using an assay validated using Chinese hamster ovary (CHO) cells and a known genotoxicant, mitomycin C. Subsequently, the assay was developed for the human peripheral blood lymphocyte (HPBL) system, as it was felt that results derived from human cells would reflect the situation more closely in vivo. Condensates from Southern yellow pine, Eastern white pine and Douglas fir trees were tested in CHO and HPBL systems and have demonstrated cytotoxic and genotoxic effects in vitro, as reported elsewhere. Red oak condensate has also been tested using the HPBL system. Thus far, results are consistent with the hypothesis that there is no difference between the cytotoxic and genotoxic effects of treated cells versus controls. This finding indicates either that the condensate of red oak poses no appreciable genetic hazard as measured by cytotoxicity and genotoxicity assays, or that the condensate has lost its potency with time and storage; both of these possibilities have important environmental implications.
木材工业的一项主要活动是木材的窑干。为了确定木材干燥冷凝物是否会对环境造成潜在危害，使用经中国仓鼠卵巢（CHO）细胞和已知基因毒性剂丝裂霉素C验证的试验，对这些冷凝物在体外的细胞毒性和基因毒性进行了测试。随后，针对人类外周血淋巴细胞（HPBL）系统开发了该试验，因为人们认为来自人类细胞的结果将更能反映体内的实际情况。对南部黄松、东部白松和花旗松树的冷凝物在CHO和HPBL系统中进行了测试，并已在体外显示出细胞毒性和基因毒性作用，其他地方也有相关报道。红橡木冷凝物也已使用HPBL系统进行了测试。到目前为止，结果与以下假设一致，即处理过的细胞与对照细胞在细胞毒性和基因毒性作用方面没有差异。这一发现表明，要么红橡木冷凝物通过细胞毒性和基因毒性试验测量不会造成明显的遗传危害，要么冷凝物随着时间和储存已经失去了效力；这两种可能性都具有重要的环境意义。
