Xu W, Li H, Brodniewicz T, Auger F A, Germain L
Laboratoire de Recherche des Grands Brûlés/LOEX, Hôpital du Saint-Sacrement, Québec, Canada.
Burns. 1996 May;22(3):191-6. doi: 10.1016/0305-4179(95)00124-7.
Grafting of cultured epidermal sheets is a promising technique for skin restoration in extensive burns, but the technique has some limitations, resulting in variable graft takes. These experiments were designed to evaluate the innocuity of Hemaseel HMN fibrin sealant in the grafting process and in vivo evolution of cultured epidermis. A total of 30 mice were grafted, 15 were controls, 15 received tissue sealant application before the deposition of the cultured human epidermal sheets. Seven days after transplantation, compared to controls, the percentage of graft take over the total surface area grafted was greater in animals that had received the tissue sealant application. No difference was found 14 and 21 days postgrafting. In contrast, the percentage of graft take over the bony area (spinal) was significantly increased in animals grafted with previous application of sealant compared to controls at 7, 14 and 21 days postgrafting. Immunohistological and ultrastructural analysis showed that the evolution of the cultured human epidermis after transplantation was similar in both groups. The basement membrane was well structured 21 days after transplantation. The sealant was present at 4 days but not at 21 days postgrafting. Therefore, we conclude that the application of fibrin sealant before cultured epidermal sheet deposition on nude mouse graft bed is innocuous and enhances their mechanical stability. Since in this nude mouse system Hemaseel HMN fibrin sealant increased the percentage of graft take over areas difficult to engraft, we think that it may be advantageous in cultured epidermal sheet grafting on burn patients.
培养表皮片移植是广泛烧伤皮肤修复的一种有前景的技术，但该技术存在一些局限性，导致移植成活率各异。这些实验旨在评估Hemaseel HMN纤维蛋白密封剂在移植过程中以及培养表皮在体内演变过程中的安全性。总共对30只小鼠进行了移植，15只为对照组，15只在植入培养的人表皮片之前应用了组织密封剂。移植后7天，与对照组相比，应用了组织密封剂的动物移植成活面积占总移植面积的百分比更高。移植后14天和21天未发现差异。相反，在移植后7天、14天和21天，与对照组相比，预先应用密封剂的移植动物在骨区（脊柱）的移植成活百分比显著增加。免疫组织学和超微结构分析表明，两组移植后培养的人表皮演变相似。移植后21天基底膜结构良好。密封剂在移植后4天存在，但在21天时不存在。因此，我们得出结论，在裸鼠移植床上植入培养表皮片之前应用纤维蛋白密封剂是无害的，并能增强其机械稳定性。由于在这个裸鼠系统中，Hemaseel HMN纤维蛋白密封剂增加了在难以植入区域的移植成活百分比，我们认为它在烧伤患者的培养表皮片移植中可能具有优势。
