Hashino S, Imamura M, Tanaka J, Kasai M, Higa T, Asaka M
Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Leuk Lymphoma. 1996 Apr;21(3-4):331-7. doi: 10.3109/10428199209067616.
We investigated the possibility for induction of graft-versus-tumor (GVT) effects in cyclosporine A (CsA)- or FK506 (FK)-treated DBA/2 mice after syngeneic bone marrow transplantation (BMT). For in vitro assays of spleen cells, the CsA-treated mice had more enhanced cytotoxic activity against YAC1 and P388, while the FK-treated animals had more against P815, YAC1, and P388. IL-4 mRNA expression was detected in spleen cells of the FK-treated mice and IL-6 mRNA expression was clearly detected in both the treated groups. Concerning GVT effects, FK had more pronounced immunostimulatory potential than CsA in this experimental setting using DBA/2 mice. In tumor-loading in vivo experiments, we could not show any antitumor effect on survival. However, this immunostimulation could be expected to eradicate the minimal residual disease after autologous BMT and autologous peripheral blood stem cell transplantation.
我们研究了在同基因骨髓移植（BMT）后，用环孢素A（CsA）或FK506（FK）处理的DBA/2小鼠中诱导移植物抗肿瘤（GVT）效应的可能性。对于脾细胞的体外检测，用CsA处理的小鼠对YAC1和P388具有更强的细胞毒活性，而用FK处理的动物对P815、YAC1和P388具有更强的细胞毒活性。在FK处理的小鼠脾细胞中检测到IL-4 mRNA表达，并且在两个处理组中均清楚地检测到IL-6 mRNA表达。关于GVT效应，在使用DBA/2小鼠的该实验设置中，FK比CsA具有更明显的免疫刺激潜力。在体内肿瘤负荷实验中，我们未能显示出对生存的任何抗肿瘤作用。然而，这种免疫刺激有望根除自体BMT和自体外周血干细胞移植后的微小残留病。
