Kohsa K, Yamada H, Sugihara H
Department of Pathology, Saga Medical School, Japan.
Cell Struct Funct. 1996 Feb;21(1):81-9. doi: 10.1247/csf.21.81.
We cultured isolated alveolar type II epithelial cells on a collagen gel matrix. At confluence, cultured type II cells were exposed to air. Under these conditions, the cellular density of the type II cells increased and they nodularly aggregated. The cultured cells consisted mainly of flattened epithelia intermingled with cuboidal cells. In the cytoplasm and at the apical surface of cuboidal cells, a surfactant protein was detected by immunohistochemistry and electron microscopy. Electron microscopic examination revealed that the surfactants in the cytoplasm increased when the cells were exposed to air. On the other hand, the flattened cells morphologically resembled type I cells in vivo. Air exposure treatment on the collagen gel matrix promoted the maintenance of the characteristic differentiation of alveolar epithelial cells. This culture system seemed to provide an appropriate physiological environment in which to study differentiation and disorders of pulmonary alveoli.
我们将分离出的肺泡II型上皮细胞培养在胶原凝胶基质上。汇合时，将培养的II型细胞暴露于空气中。在这些条件下，II型细胞的细胞密度增加并形成结节状聚集。培养的细胞主要由扁平上皮细胞与立方形细胞混合组成。通过免疫组织化学和电子显微镜检测到，在立方形细胞的细胞质和顶端表面存在一种表面活性蛋白。电子显微镜检查显示，当细胞暴露于空气中时，细胞质中的表面活性剂增加。另一方面，扁平细胞在形态上类似于体内的I型细胞。在胶原凝胶基质上进行空气暴露处理促进了肺泡上皮细胞特征性分化的维持。这种培养系统似乎提供了一个合适的生理环境，可用于研究肺泡的分化和疾病。
