Bingle L, Bull T B, Fox B, Guz A, Richards R J, Tetley T D
Department of Medicine, Charing Cross and Westminster Medical School, London, UK.
Environ Health Perspect. 1990 Apr;85:71-80. doi: 10.1289/ehp.85-1568322.
Alveolar Type II epithelial cells dedifferentiate rapidly in vitro. Studies with animal tissue suggest that cell-cell and extracellular matrix-cell interactions are important in the retention of Type II cell morphology in vitro. Thus, in this study with human tissue, alveolar Type II cells, alveolar macrophages, and spindle cells were prepared from the same sample of lung (obtained following lobectomy for cancer, n = 3), cocultured on glass cover slips or tissue culture plastic, and studied by light microscopy with scanning (SEM) and transmission (TEM) electron microscopy for 8 days. The primary cell isolates contained approximately 45% Type II cells; the remainder were macrophages or unidentifiable cells. Clusters, made up of a single layer of cuboidal Type II cells around a central core of connective tissue (largely collagen and some elastic tissue), formed above a monolayer of spindle cells. The Type II cells were morphologically similar to those seen in vivo. The cells were still cuboidal at 8 days but had lost their lamellar bodies, which were released into the medium via the apical surface. The clusters increased in size with time (area, microns 2: day 1, 29(5-143) x 10(2); day 8, 63(10-311) x 10(2); mean(range); p less than 0.02) without changing in number per culture, suggesting Type II cell proliferation. This may have been due to factors produced by the other cells and adherence to the extracellular matrix (ECM); (free collagen fibers, present in the original preparation, spindle cells, and/or Type II cells could be responsible for presence of ECM). We propose this as a useful model for the study of human Type II epithelial cells in vitro.
肺泡II型上皮细胞在体外迅速去分化。对动物组织的研究表明，细胞间和细胞外基质与细胞的相互作用对于在体外保持II型细胞形态很重要。因此，在这项针对人体组织的研究中，从同一肺样本（因癌症行肺叶切除术后获得，n = 3）中制备了肺泡II型细胞、肺泡巨噬细胞和梭形细胞，将它们共培养在玻璃盖玻片或组织培养塑料上，并通过光学显微镜、扫描电子显微镜（SEM）和透射电子显微镜（TEM）研究8天。原代细胞分离物中约含45%的II型细胞；其余为巨噬细胞或无法识别的细胞。在单层梭形细胞上方形成了由围绕结缔组织（主要是胶原蛋白和一些弹性组织）中央核心的单层立方形II型细胞组成的细胞簇。II型细胞在形态上与体内所见的相似。这些细胞在第8天时仍为立方形，但已失去板层小体，板层小体通过顶端表面释放到培养基中。细胞簇的大小随时间增加（面积，平方微米：第1天，29(5 - 143)×10²；第8天，63(10 - 311)×10²；平均值（范围）；p < 0.02），而每个培养物中的细胞数量不变，提示II型细胞增殖。这可能是由于其他细胞产生的因子以及对细胞外基质（ECM）的黏附所致；（原始制剂中存在的游离胶原纤维、梭形细胞和/或II型细胞可能是ECM存在的原因）。我们认为这是一个用于体外研究人II型上皮细胞的有用模型。
