Peshwa M V, Wu F J, Sharp H L, Cerra F B, Hu W S
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis 55455-0132, USA.
In Vitro Cell Dev Biol Anim. 1996 Apr;32(4):197-203. doi: 10.1007/BF02722946.
Freshly harvested rat hepatocytes form spheroids on uncoated positively charged polystyrene surfaces. Time lapse microscopy revealed that cell movement and reorganization were involved in spheroid formation. Ultrastructural evaluation using scanning and transmission electron microscopy indicated polarized cellular morphology and extensive cell-cell communication within spheroids. Bile canalicular structures were observed to surround each individual hepatocyte, forming an intricate three-dimensional continuous network of channels that appeared to end as pores/holes on the surface of the spheroid. The maintenance of differentiated cellular morphology coincided with preservation of hepatocyte viability and enhanced levels of tissue specific functions in spheroids.
新鲜收获的大鼠肝细胞在未包被的带正电荷聚苯乙烯表面形成球体。延时显微镜观察显示，细胞运动和重组参与了球体形成。使用扫描电子显微镜和透射电子显微镜进行的超微结构评估表明，球体内部细胞形态呈极化状态，且细胞间存在广泛的通讯。观察到胆小管结构围绕着每个单独的肝细胞，形成了一个复杂的三维连续通道网络，这些通道似乎在球体表面以孔隙/孔洞的形式终止。分化细胞形态的维持与肝细胞活力的保持以及球体中组织特异性功能水平的提高相一致。
