Pongsuwanna Y, Taniguchi K, Chiwakul M, Urasawa T, Wakasugi F, Jayavasu C, Urasawa S
Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
J Clin Microbiol. 1996 May;34(5):1050-7. doi: 10.1128/jcm.34.5.1050-1057.1996.
A total of 557 fecal specimens collected from piglets with diarrhea in Thailand were examined for rotavirus RNA by polyacrylamide gel electrophoresis. Twenty-three, one, and two samples were positive for group A, group B, and group C rotaviruses, respectively. Two samples exhibited two segments found in picobirnavirus RNA. RNA electropherotyping of 23 group A rotaviruses showed that they were classified into five patterns. By serotyping by enzyme-linked immunosorbent assay and PCR, viruses in 3 and 14 specimens were found to be serotype G3 and serotype G10, respectively. For one specimen, containing a serotype G10 virus (strain P343), virus was isolated in MA-104 cells, and the nucleotide sequences of the VP7 and VP4 genes were determined. Comparative sequence analysis and cross-neutralization tests showed that strain P343 has B223-like G10 and UK-like P7 serotype (or VP4 genotype 5) specificities. Rotaviruses having such antigenic specificities have not been detected in piglets. Thus, the interspecies transmission of rotaviruses between cows and pigs was suggested.
对从泰国腹泻仔猪采集的557份粪便样本进行聚丙烯酰胺凝胶电泳检测轮状病毒RNA。分别有23份、1份和2份样本对A组、B组和C组轮状病毒呈阳性。两份样本显示出微小双RNA病毒RNA中的两个片段。对23株A组轮状病毒进行RNA电泳分型，结果表明它们可分为五种模式。通过酶联免疫吸附测定和PCR进行血清分型，发现3份样本中的病毒为G3血清型，14份样本中的病毒为G10血清型。对于一份含有G10血清型病毒（P343株）的样本，在MA - 104细胞中分离出病毒，并测定了VP7和VP4基因的核苷酸序列。比较序列分析和交叉中和试验表明，P343株具有B223样G10和UK样P7血清型（或VP4基因型5）特异性。在仔猪中尚未检测到具有这种抗原特异性的轮状病毒。因此，提示轮状病毒在牛和猪之间存在种间传播。
