Marcantonio J M
Biomedical Centre, School of Biological Sciences, University of East Anglia, Norwich, UK.
Ophthalmic Res. 1996;28 Suppl 1:48-50. doi: 10.1159/000267943.
Ionic homeostasis is essential to lens clarity and the lens epithelium plays a large part in homeostasis, through vectorial transport. In most epithelia maintenance of vectorial function depends on the cytoskeleton. Capsule/epithelium preparations from human donor lenses have been used for immunohistochemical investigations of both normal epithelial cell cytoskeletal structure, and of structural changes induced by increasing cell calcium. A sustained increase in intracellular calcium, induced by incubation with A23187 and W7, led to a loss of cytoskeletal organisation. Changes induced depolymerisation of cytoplasmic actin filaments, disaggregation of microtubules and initial thickening, followed by breakdown, of vimentin intermediate filaments. In addition, spectrin staining, normally confined to the basal-lateral plasma membranes, became diffuse within the cytoplasm. Such disruptions of cytoskeletal structure would decrease ionic control, leading to increasing osmotic stress.
离子稳态对于晶状体的透明度至关重要，而晶状体上皮细胞通过矢量运输在稳态维持中发挥着重要作用。在大多数上皮细胞中，矢量功能的维持依赖于细胞骨架。来自人类供体晶状体的囊膜/上皮细胞制剂已被用于对正常上皮细胞细胞骨架结构以及细胞钙增加所诱导的结构变化进行免疫组织化学研究。用A23187和W7孵育诱导细胞内钙持续增加，导致细胞骨架组织丧失。这些变化引起细胞质肌动蛋白丝解聚、微管解聚以及波形蛋白中间丝最初增厚，随后断裂。此外，血影蛋白染色通常局限于基底外侧质膜，在细胞质内变得弥散。细胞骨架结构的这种破坏会降低离子控制，导致渗透应激增加。
