Duindam J J, Vrensen G F, Otto C, Greve J
Netherlands Ophthalmic Research Institute, Department of Morphology, Amsterdam, The Netherlands.
Ophthalmic Res. 1996;28 Suppl 1:86-91. doi: 10.1159/000267978.
The distribution of 3-beta-OH-cholesterol along the optical axis of 20 human eye lenses of different ages was measured using confocal Raman microspectroscopy in combination with the 3-beta-hydroxysteroid-specific probe filipin. This non-destructive technique allow a spatially very detailed study of the distribution of 3-beta-OH-cholesterol in individual lenses. It revealed age-related changes of the 3-beta-OH-cholesterol distribution along the optical axis. The 3-beta-OH-cholesterol was found to be distributed asymmetrically along the optical axis and this distribution was found to change with age. The relative 3-beta-OH-cholesterol content in the anterior part of the lens decreases significantly with increasing age compared with its equal posterior counterpart. Additionally all the lenses have a very low 3-beta-OH-cholesterol content in the central part of their nuclear region, the area where the primary lens fibres are located.
使用共聚焦拉曼显微光谱法结合3-β-羟基类固醇特异性探针制霉菌素，测量了20个不同年龄人眼晶状体沿光轴的3-β-羟基胆固醇分布。这种无损技术能够对单个晶状体中3-β-羟基胆固醇的分布进行空间上非常详细的研究。它揭示了沿光轴的3-β-羟基胆固醇分布与年龄相关的变化。发现3-β-羟基胆固醇沿光轴不对称分布，并且这种分布随年龄变化。与晶状体后部相同部位相比，晶状体前部的相对3-β-羟基胆固醇含量随年龄增长显著降低。此外，所有晶状体在其核区域的中心部分（即初级晶状体纤维所在区域）的3-β-羟基胆固醇含量都非常低。
