Chen N T, Butler P E, Hooper D C, May J W
Department of Surgery, Massachusetts General Hospital, Boston 02114, USA.
Ann Plast Surg. 1996 Apr;36(4):337-41. doi: 10.1097/00000637-199604000-00001.
The survival of bacteria was evaluated in custom-made saline breast implants with integral injection ports in vitro and in 10 New Zealand White rabbits for Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Serratia marcescens. Pseudomonas and Serratia survived in vitro in saline-filled implants and multiplied 24-fold and 22-fold, respectively, from the initial inocula of 300 colony-forming units per cubic centimeter in 21 days. Serratia alone survived in saline implants placed on the dorsum of rabbits, proliferated 80-fold in 7 days, and tapered to 10-fold at the end of 3 weeks. Chemical analysis revealed the presence of glucose in fluid from the implants in the animal study (mean, 1.2 mg per deciliter; standard error of mean [SEM], 0.6) after 21 days and from human subjects (mean, 3.8 mg per deciliter; SEM, 1.0) after 8 months to 10 years. Serratia incubated in human breast implant fluid samples proliferated 7-fold to 30-fold greater than in the saline control in a nonaerated environment. We conclude that some bacteria are able to proliferate in saline in breast implants. Furthermore, their survival may be facilitated by a substance (i.e., glucose) that diffuses across the implant outer shell.
在定制的带有整体注射端口的盐水乳房植入物中，对金黄色葡萄球菌、表皮葡萄球菌、铜绿假单胞菌和粘质沙雷氏菌进行了体外以及在10只新西兰白兔体内的细菌存活情况评估。铜绿假单胞菌和粘质沙雷氏菌在体外充满盐水的植入物中存活，在21天内分别从每立方厘米300个菌落形成单位的初始接种量繁殖了24倍和22倍。仅粘质沙雷氏菌在置于兔背部的盐水植入物中存活，7天内增殖了80倍，在3周结束时降至10倍。化学分析显示，在动物研究中，21天后植入物液体中存在葡萄糖（平均每分升1.2毫克；平均标准误差[SEM]为0.6），在人体研究中，8个月至10年后葡萄糖含量为平均每分升3.8毫克（SEM为1.0）。在非充气环境中，在人乳房植入物液体样本中培养的粘质沙雷氏菌比在盐水对照中增殖7倍至30倍。我们得出结论，一些细菌能够在乳房植入物的盐水中增殖。此外，它们的存活可能因一种扩散穿过植入物外壳的物质（即葡萄糖）而得到促进。
