Zheng Y, Liu D, Feng D, Tang H, Li Y, You X
Second Affiliated Hospital, Hunan Medical University, Changsha.
Chin Med J (Engl). 1995 Dec;108(12):895-7.
Three gena of mosquitoes were fed with human blood infected with hepatitis B virus (HBV) artificially. When blood in the stomach was completely digested, mosquitoes were used to bite monkeys Tupaia Belangeri. A month after biting, venous blood samples were taken from the monkeys to detect serum HBsAg, HBeAg, Anti-HBs and Anti-HBc. Among 29 test monkeys, 9 were HBV infective serum markers positive. In 4 of 16 test monkey liver biopsies, acute inflammatory changes were found with Hematoxylin-Eosine staining. In the hepatic cells, HBsAg and HBcAg were detected by immunohistochemical double-labeling assays, and HBV-DNA was also positive by in situ hybridization in monkey liver sections. These results provided an evidence for the possibility of transmission of HBV through mosquitoes and suggest its epidemiological significance in mosquito infested areas.
用人工感染乙型肝炎病毒（HBV）的人血喂养三只基因类型的蚊子。当胃中的血液完全消化后，用这些蚊子叮咬树鼩（Tupaia Belangeri）。叮咬一个月后，从树鼩采集静脉血样本，检测血清HBsAg、HBeAg、抗 - HBs和抗 - HBc。在29只受试树鼩中，9只的HBV感染血清标志物呈阳性。在16只受试树鼩的肝脏活检中，4只经苏木精 - 伊红染色发现有急性炎症变化。通过免疫组织化学双标记检测在肝细胞中检测到HBsAg和HBcAg，并且在树鼩肝脏切片中通过原位杂交检测HBV - DNA也呈阳性。这些结果为HBV通过蚊子传播的可能性提供了证据，并表明其在蚊虫滋生地区的流行病学意义。
