Sarsfield P, Rinne A, Jones D B, Johnson P, Wright D H
Department of Pathology, Southampton General Hospital, UK.
Histopathology. 1996 Mar;28(3):205-11. doi: 10.1046/j.1365-2559.1996.d01-417.x.
We report a study of the organization of accessory cell populations, in normal mucosal lymphoid tissue from small intestine (8 cases), large intestine (6) and appendix (9) using a panel of monoclonal antibodies and polyclonal antisera in paraffin-embedded tissue. Two populations were identified in dome areas, one positive for acid cysteine proteinase inhibitor and HLA class II (WR18) only and the second positive for S-100 protein, CD68, and WR18 and negative for acid cysteine proteinase inhibitor and factor XIIIa. Superficial colonic mucosal and small intestinal villous tip macrophages stained positively with CD68 and WR18 only, while deeper cryptal and submucosal populations exhibited additional positivity for factor XIIIa, but both populations were negative for acid cysteine proteinase inhibitor and S-100 protein. Germinal centre macrophages were positive for CD68, WR18 and acid cysteine proteinase inhibitor and negative for factor XIIIa, and S-100 protein. T zone dendritic cells included a population which stained positively for S-100 protien, WR18 and were negative for factor XIIIa, CD68 and acid cysteine proteinase inhibitor, an immunophenotype typical of interdigitating dendritic reticulum cells. This distribution of phenotypically identifiable accessory cell subpopulations was apparent at all three sites examined. We suggest that the specialized subpopulations of dendritic cells staining for S-100 protein and for acid cysteine proteinase inhibitor which are restricted to the dome areas, may have a potential role in the transfer of antigen across the epithelium to the germinal centres, while factor XIIIa appears to identify a tissue macrophage population with a potential role in stromal modulation distant from direct antigen challenge.
我们报告了一项关于辅助细胞群体组织的研究，该研究使用一组单克隆抗体和多克隆抗血清，对石蜡包埋组织中的小肠（8例）、大肠（6例）和阑尾（9例）的正常黏膜淋巴组织进行了检测。在圆顶区域鉴定出两种细胞群体，一种仅对酸性半胱氨酸蛋白酶抑制剂和HLA II类分子（WR18）呈阳性，另一种对S-100蛋白、CD68和WR18呈阳性，而对酸性半胱氨酸蛋白酶抑制剂和因子XIIIa呈阴性。结肠黏膜浅层和小肠绒毛顶端的巨噬细胞仅对CD68和WR18呈阳性染色，而更深层的隐窝和黏膜下细胞群体对因子XIIIa呈额外阳性，但这两种细胞群体对酸性半胱氨酸蛋白酶抑制剂和S-100蛋白均呈阴性。生发中心巨噬细胞对CD68、WR18和酸性半胱氨酸蛋白酶抑制剂呈阳性，而对因子XIIIa和S-100蛋白呈阴性。T区树突状细胞包括一群对S-100蛋白、WR18呈阳性染色，而对因子XIIIa、CD68和酸性半胱氨酸蛋白酶抑制剂呈阴性的细胞，这种免疫表型是交错突树突状网状细胞的典型特征。在所检查的所有三个部位，表型可识别的辅助细胞亚群的这种分布都是明显的。我们认为，仅局限于圆顶区域的、对S-100蛋白和酸性半胱氨酸蛋白酶抑制剂呈阳性染色的树突状细胞特殊亚群，可能在抗原跨上皮转运至生发中心的过程中具有潜在作用，而因子XIIIa似乎可识别出一群在远离直接抗原刺激的基质调节中具有潜在作用的组织巨噬细胞。
